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| | ==Crystal structures and site-directed mutagenesis study of nitroalkane oxidase from Streptomyces ansochromogenes== | | ==Crystal structures and site-directed mutagenesis study of nitroalkane oxidase from Streptomyces ansochromogenes== |
| - | <StructureSection load='3bw3' size='340' side='right' caption='[[3bw3]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='3bw3' size='340' side='right'caption='[[3bw3]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3bw3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_ansochromogenes"_yen_and_zhang_1964 "actinomyces ansochromogenes" yen and zhang 1964]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BW3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BW3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3bw3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_ansochromogenes Streptomyces ansochromogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BW3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BW3 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NIE:NITROETHANE'>NIE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3bw2|3bw2]], [[3bw4|3bw4]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NIE:NITROETHANE'>NIE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">2-npdl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=115647 "Actinomyces ansochromogenes" Yen and Zhang 1964])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bw3 OCA], [https://pdbe.org/3bw3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bw3 RCSB], [https://www.ebi.ac.uk/pdbsum/3bw3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bw3 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitroalkane_oxidase Nitroalkane oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.1 1.7.3.1] </span></td></tr> | + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bw3 OCA], [http://pdbe.org/3bw3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bw3 RCSB], [http://www.ebi.ac.uk/pdbsum/3bw3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bw3 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9FDD4_9ACTN Q9FDD4_9ACTN] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Actinomyces ansochromogenes yen and zhang 1964]] | + | [[Category: Large Structures]] |
| - | [[Category: Nitroalkane oxidase]] | + | [[Category: Streptomyces ansochromogenes]] |
| - | [[Category: Gao, Z Q]] | + | [[Category: Gao ZQ]] |
| - | [[Category: Hou, H F]] | + | [[Category: Hou HF]] |
| - | [[Category: Li, Y H]] | + | [[Category: Li YH]] |
| - | [[Category: Dioxygenase]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Tim barrel]]
| + | |
| Structural highlights
Function
Q9FDD4_9ACTN
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Nitroalkane oxidase (NAO) catalyzes neutral nitroalkanes to their corresponding aldehydes or ketones, hydrogen peroxide and nitrite. The crystal structure of NAO from Streptomyces ansochromogenes was determined; it consists of two domains, a TIM barrel domain bound to FMN and C-terminal domain with a novel folding pattern. Site-directed mutagenesis of His179, which is spatially adjacent to FMN, resulted in the loss of enzyme activity, demonstrating that this amino acid residue is important for catalysis. The crystal structure of mutant H179D-nitroethane was also analyzed. Interestingly, Sa-NAO shows the typical function as nitroalkane oxidase but its structure is similar to that of 2-nitropropane dioxygenase. Overall, these results suggest that Sa-NAO is a novel nitroalkane oxidase with TIM barrel structure.
Crystal structure and site-directed mutagenesis of a nitroalkane oxidase from Streptomyces ansochromogenes.,Li Y, Gao Z, Hou H, Li L, Zhang J, Yang H, Dong Y, Tan H Biochem Biophys Res Commun. 2011 Feb 18;405(3):344-8. Epub 2010 Dec 11. PMID:21147069[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Li Y, Gao Z, Hou H, Li L, Zhang J, Yang H, Dong Y, Tan H. Crystal structure and site-directed mutagenesis of a nitroalkane oxidase from Streptomyces ansochromogenes. Biochem Biophys Res Commun. 2011 Feb 18;405(3):344-8. Epub 2010 Dec 11. PMID:21147069 doi:10.1016/j.bbrc.2010.12.050
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