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| | ==Crystal Structure of MarR== | | ==Crystal Structure of MarR== |
| - | <StructureSection load='3bpx' size='340' side='right' caption='[[3bpx]], [[Resolution|resolution]] 1.95Å' scene=''> | + | <StructureSection load='3bpx' size='340' side='right'caption='[[3bpx]], [[Resolution|resolution]] 1.95Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3bpx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanobacterium_thermoautotrophicum Methanobacterium thermoautotrophicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BPX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BPX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3bpx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_marburgensis_str._Marburg Methanothermobacter marburgensis str. Marburg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BPX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BPX FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3bpv|3bpv]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MT313 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=145262 Methanobacterium thermoautotrophicum])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bpx OCA], [https://pdbe.org/3bpx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bpx RCSB], [https://www.ebi.ac.uk/pdbsum/3bpx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bpx ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bpx OCA], [http://pdbe.org/3bpx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bpx RCSB], [http://www.ebi.ac.uk/pdbsum/3bpx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bpx ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/O26413_METTH O26413_METTH] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Methanobacterium thermoautotrophicum]] | + | [[Category: Large Structures]] |
| - | [[Category: Christendat, D]] | + | [[Category: Methanothermobacter marburgensis str. Marburg]] |
| - | [[Category: Saridakis, V]] | + | [[Category: Christendat D]] |
| - | [[Category: Shahinas, D]] | + | [[Category: Saridakis V]] |
| - | [[Category: Xu, X]] | + | [[Category: Shahinas D]] |
| - | [[Category: Dna binding]]
| + | [[Category: Xu X]] |
| - | [[Category: Dna-binding]]
| + | |
| - | [[Category: Marr]]
| + | |
| - | [[Category: Transcription factor]]
| + | |
| - | [[Category: Transcription regulation]]
| + | |
| - | [[Category: Transcription regulator]]
| + | |
| - | [[Category: Winged helix motif]]
| + | |
| Structural highlights
Function
O26413_METTH
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Transcriptional regulators belonging to the MarR family are characterized by a winged-helix DNA binding domain. These transcriptional regulators regulate the efflux and influx of phenolic agents in bacteria and archaea. In Escherichia coli, MarR regulates the multiple antibiotic resistance operon and its inactivation produces a multiple antibiotic resistance phenotype. In some organisms, active efflux of drug compounds will produce a drug resistance phenotype, whereas in other organisms, active influx of chlorinated hydrocarbons results in their rapid degradation. Although proteins in the MarR family are regulators of important biological processes, their mechanism of action is not well understood and structural information about how phenolic agents regulate the activity of these proteins is lacking. This article presents the three-dimensional structure of a protein of the MarR family, MTH313, in its apo form and in complex with salicylate, a known inactivator. A comparison of these two structures indicates that the mechanism of regulation involves a large conformational change in the DNA binding lobe. Electrophoretic mobility shift assay and biophysical analyses further suggest that salicylate inactivates MTH313 and prevents it from binding to its promoter region.
Structural insight on the mechanism of regulation of the MarR family of proteins: high-resolution crystal structure of a transcriptional repressor from Methanobacterium thermoautotrophicum.,Saridakis V, Shahinas D, Xu X, Christendat D J Mol Biol. 2008 Mar 28;377(3):655-67. Epub 2008 Jan 11. PMID:18272181[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Saridakis V, Shahinas D, Xu X, Christendat D. Structural insight on the mechanism of regulation of the MarR family of proteins: high-resolution crystal structure of a transcriptional repressor from Methanobacterium thermoautotrophicum. J Mol Biol. 2008 Mar 28;377(3):655-67. Epub 2008 Jan 11. PMID:18272181 doi:10.1016/j.jmb.2008.01.001
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