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| | ==Crystal structure of the P. putida toluene transporter TodX== | | ==Crystal structure of the P. putida toluene transporter TodX== |
| - | <StructureSection load='3bs0' size='340' side='right' caption='[[3bs0]], [[Resolution|resolution]] 2.60Å' scene=''> | + | <StructureSection load='3bs0' size='340' side='right'caption='[[3bs0]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3bs0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BS0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3bs0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida_F1 Pseudomonas putida F1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BS0 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3bry|3bry]], [[3brz|3brz]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">todX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 Pseudomonas putida])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bs0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bs0 OCA], [https://pdbe.org/3bs0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bs0 RCSB], [https://www.ebi.ac.uk/pdbsum/3bs0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bs0 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bs0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bs0 OCA], [http://pdbe.org/3bs0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bs0 RCSB], [http://www.ebi.ac.uk/pdbsum/3bs0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bs0 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A5W4F4_PSEP1 A5W4F4_PSEP1] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Pseudomonas putida]] | + | [[Category: Large Structures]] |
| - | [[Category: Berg, B van den]] | + | [[Category: Pseudomonas putida F1]] |
| - | [[Category: Hearn, E M]] | + | [[Category: Hearn EM]] |
| - | [[Category: Patel, D R]] | + | [[Category: Patel DR]] |
| - | [[Category: Beta barrel]] | + | [[Category: Van den Berg B]] |
| - | [[Category: Outer membrane protein]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
A5W4F4_PSEP1
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Bacterial biodegradation of hydrocarbons, an important process for environmental remediation, requires the passage of hydrophobic substrates across the cell membrane. Here, we report crystal structures of two outer membrane proteins, Pseudomonas putida TodX and Ralstonia pickettii TbuX, which have been implicated in hydrocarbon transport and are part of a subfamily of the FadL fatty acid transporter family. The structures of TodX and TbuX show significant differences with those previously determined for Escherichia coli FadL, which may provide an explanation for the substrate-specific transport of TodX and TbuX observed with in vivo transport assays. The TodX and TbuX structures revealed 14-stranded beta-barrels with an N-terminal hatch domain blocking the barrel interior. A hydrophobic channel with bound detergent molecules extends from the extracellular surface and is contiguous with a passageway through the hatch domain, lined by both hydrophobic and polar or charged residues. The TodX and TbuX structures support a mechanism for transport of hydrophobic substrates from the extracellular environment to the periplasm via a channel through the hatch domain.
Outer-membrane transport of aromatic hydrocarbons as a first step in biodegradation.,Hearn EM, Patel DR, van den Berg B Proc Natl Acad Sci U S A. 2008 Jun 24;105(25):8601-6. Epub 2008 Jun 16. PMID:18559855[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hearn EM, Patel DR, van den Berg B. Outer-membrane transport of aromatic hydrocarbons as a first step in biodegradation. Proc Natl Acad Sci U S A. 2008 Jun 24;105(25):8601-6. Epub 2008 Jun 16. PMID:18559855
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