3bje

<StructureSection load='3bje' size='340' side='right' caption='[[3bje]], [[Resolution|resolution]] 1.44&Aring;' scene=''>

<table><tr><td colspan='2'>[[3bje]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_(trypanozoon)_brucei Trypanosoma (trypanozoon) brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BJE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BJE FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=R1P:RIBOSE-1-PHOSPHATE'>R1P</scene>, <scene name='pdbligand=URA:URACIL'>URA</scene></td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tb927.8.4430 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5691 Trypanosoma (Trypanozoon) brucei])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bje OCA], [http://pdbe.org/3bje PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bje RCSB], [http://www.ebi.ac.uk/pdbsum/3bje PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bje ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Purine nucleoside phosphorylases and uridine phosphorylases (UPs) are closely related enzymes involved in purine and pyrimidine salvage, respectively, which catalyze the removal of the ribosyl moiety from nucleosides so that the nucleotide base may be recycled. Parasitic protozoa generally are incapable of de novo purine biosynthesis; hence, the purine salvage pathway is of potential therapeutic interest. Information about pyrimidine biosynthesis in these organisms is much more limited. Though all seem to carry at least a subset of enzymes from each pathway, the dependency on de novo pyrimidine synthesis versus salvage varies from organism to organism and even from one growth stage to another. We have structurally and biochemically characterized a putative nucleoside phosphorylase (NP) from the pathogenic protozoan Trypanosoma brucei and find that it is a homodimeric UP. This is the first characterization of a UP from a trypanosomal source despite this activity being observed decades ago. Although this gene was broadly annotated as a putative NP, it was widely inferred to be a purine nucleoside phosphorylase. Our characterization of this trypanosomal enzyme shows that it is possible to distinguish between purine and UP activity at the sequence level based on the absence or presence of a characteristic UP-specificity insert. We suggest that this recognizable feature may aid in proper annotation of the substrate specificity of enzymes in the NP family.

The Crystal Structure and Activity of a Putative Trypanosomal Nucleoside Phosphorylase Reveal It to be a Homodimeric Uridine Phosphorylase.,Larson ET, Mudeppa DG, Gillespie JR, Mueller N, Napuli AJ, Arif JA, Ross J, Arakaki TL, Lauricella A, Detitta G, Luft J, Zucker F, Verlinde CL, Fan E, Van Voorhis WC, Buckner FS, Rathod PK, Hol WG, Merritt EA J Mol Biol. 2010 Jan 11. PMID:20070944<ref>PMID:20070944</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 3bje" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Larson, E T]]

[[Category: Merritt, E A]]

[[Category: Structural genomic]]

[[Category: Brucei]]

[[Category: Uridine phosphorylase]]