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6i27

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'''Unreleased structure'''
 
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The entry 6i27 is ON HOLD
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==Rea1 AAA2L-H2alpha deletion mutant in AMPPNP State==
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<SX load='6i27' size='340' side='right' viewer='molstar' caption='[[6i27]], [[Resolution|resolution]] 7.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6i27]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I27 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6I27 FirstGlance]. <br>
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6hyd|6hyd]], [[6hyp|6hyp]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MDN1, REA1, YLR106C, L2901, L8004.13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6i27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i27 OCA], [http://pdbe.org/6i27 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i27 RCSB], [http://www.ebi.ac.uk/pdbsum/6i27 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i27 ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The biogenesis of 60S ribosomal subunits is initiated in the nucleus where rRNAs and proteins form pre-60S particles. These pre-60S particles mature by transiently interacting with various assembly factors. The ~5000 amino-acid AAA+ ATPase Rea1 (or Midasin) generates force to mechanically remove assembly factors from pre-60S particles, which promotes their export to the cytosol. Here we present three Rea1 cryoEM structures. We visualise the Rea1 engine, a hexameric ring of AAA+ domains, and identify an alpha-helical bundle of AAA2 as a major ATPase activity regulator. The alpha-helical bundle interferes with nucleotide-induced conformational changes that create a docking site for the substrate binding MIDAS domain on the AAA +ring. Furthermore, we reveal the architecture of the Rea1 linker, which is involved in force generation and extends from the AAA+ ring. The data presented here provide insights into the mechanism of one of the most complex ribosome maturation factors.
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Authors: Sosnowski, P., Urnavicius, L., Boland, A., Fagiewicz, R., Busselez, J., Papai, G., Schmidt, H.
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The CryoEM structure of the Saccharomyces cerevisiae ribosome maturation factor Rea1.,Sosnowski P, Urnavicius L, Boland A, Fagiewicz R, Busselez J, Papai G, Schmidt H Elife. 2018 Nov 26;7. pii: 39163. doi: 10.7554/eLife.39163. PMID:30460895<ref>PMID:30460895</ref>
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Description: Rea1 AAA2L-H2alpha deletion mutant in AMPPNP State
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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<div class="pdbe-citations 6i27" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</SX>
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[[Category: Atcc 18824]]
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[[Category: Large Structures]]
[[Category: Boland, A]]
[[Category: Boland, A]]
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[[Category: Fagiewicz, R]]
 
[[Category: Busselez, J]]
[[Category: Busselez, J]]
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[[Category: Urnavicius, L]]
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[[Category: Fagiewicz, R]]
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[[Category: Schmidt, H]]
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[[Category: Papai, G]]
[[Category: Papai, G]]
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[[Category: Schmidt, H]]
[[Category: Sosnowski, P]]
[[Category: Sosnowski, P]]
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[[Category: Urnavicius, L]]
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[[Category: Aaa+ protein]]
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[[Category: Mdn1]]
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[[Category: Midasin]]
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[[Category: Molecular machine]]
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[[Category: Motor protein]]
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[[Category: Rea1]]
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[[Category: Ribosome maturation]]

Current revision

Rea1 AAA2L-H2alpha deletion mutant in AMPPNP State

6i27, resolution 7.80Å

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