2v6e

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (01:18, 28 December 2023) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2v6e.gif|left|200px]]
 
-
{{Structure
+
==protelomerase TelK complexed with substrate DNA==
-
|PDB= 2v6e |SIZE=350|CAPTION= <scene name='initialview01'>2v6e</scene>, resolution 3.2&Aring;
+
<StructureSection load='2v6e' size='340' side='right'caption='[[2v6e]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
-
|SITE= <scene name='pdbsite=AC1:Vo4+Binding+Site+For+Chain+B'>AC1</scene>
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene>
+
<table><tr><td colspan='2'>[[2v6e]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_N15 Escherichia virus N15] and [https://en.wikipedia.org/wiki/Klebsiella_phage_phiKO2 Klebsiella phage phiKO2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V6E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V6E FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v6e OCA], [https://pdbe.org/2v6e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v6e RCSB], [https://www.ebi.ac.uk/pdbsum/2v6e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v6e ProSAT]</span></td></tr>
-
|RELATEDENTRY=
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v6e OCA], [http://www.ebi.ac.uk/pdbsum/2v6e PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2v6e RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/Q6UAV6_9CAUD Q6UAV6_9CAUD]
-
 
+
<div style="background-color:#fffaf0;">
-
'''PROTELOMERASE TELK COMPLEXED WITH SUBSTRATE DNA'''
+
== Publication Abstract from PubMed ==
-
 
+
-
 
+
-
==Overview==
+
The termini of linear chromosomes are protected by specialized DNA structures known as telomeres that also facilitate the complete replication of DNA ends. The simplest type of telomere is a covalently closed DNA hairpin structure found in linear chromosomes of prokaryotes and viruses. Bidirectional replication of a chromosome with hairpin telomeres produces a catenated circular dimer that is subsequently resolved into unit-length chromosomes by a dedicated DNA cleavage-rejoining enzyme known as a hairpin telomere resolvase (protelomerase). Here we report a crystal structure of the protelomerase TelK from Klebsiella oxytoca phage varphiKO2, in complex with the palindromic target DNA. The structure shows the TelK dimer destabilizes base pairing interactions to promote the refolding of cleaved DNA ends into two hairpin ends. We propose that the hairpinning reaction is made effectively irreversible by a unique protein-induced distortion of the DNA substrate that prevents religation of the cleaved DNA substrate.
The termini of linear chromosomes are protected by specialized DNA structures known as telomeres that also facilitate the complete replication of DNA ends. The simplest type of telomere is a covalently closed DNA hairpin structure found in linear chromosomes of prokaryotes and viruses. Bidirectional replication of a chromosome with hairpin telomeres produces a catenated circular dimer that is subsequently resolved into unit-length chromosomes by a dedicated DNA cleavage-rejoining enzyme known as a hairpin telomere resolvase (protelomerase). Here we report a crystal structure of the protelomerase TelK from Klebsiella oxytoca phage varphiKO2, in complex with the palindromic target DNA. The structure shows the TelK dimer destabilizes base pairing interactions to promote the refolding of cleaved DNA ends into two hairpin ends. We propose that the hairpinning reaction is made effectively irreversible by a unique protein-induced distortion of the DNA substrate that prevents religation of the cleaved DNA substrate.
-
==About this Structure==
+
An interlocked dimer of the protelomerase TelK distorts DNA structure for the formation of hairpin telomeres.,Aihara H, Huang WM, Ellenberger T Mol Cell. 2007 Sep 21;27(6):901-13. PMID:17889664<ref>PMID:17889664</ref>
-
2V6E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Klebsiella_phage_phiko2 Klebsiella phage phiko2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V6E OCA].
+
-
 
+
-
==Reference==
+
-
An interlocked dimer of the protelomerase TelK distorts DNA structure for the formation of hairpin telomeres., Aihara H, Huang WM, Ellenberger T, Mol Cell. 2007 Sep 21;27(6):901-13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17889664 17889664]
+
-
[[Category: Klebsiella phage phiko2]]
+
-
[[Category: Single protein]]
+
-
[[Category: Aihara, H.]]
+
-
[[Category: Ellenberger, T.]]
+
-
[[Category: Huang, W M.]]
+
-
[[Category: dna distortion]]
+
-
[[Category: hairpin telomere]]
+
-
[[Category: hydrolase]]
+
-
[[Category: protelomerase]]
+
-
[[Category: resolvase]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:08:53 2008''
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2v6e" style="background-color:#fffaf0;"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Escherichia virus N15]]
 +
[[Category: Klebsiella phage phiKO2]]
 +
[[Category: Large Structures]]
 +
[[Category: Aihara H]]
 +
[[Category: Ellenberger T]]
 +
[[Category: Huang WM]]

Current revision

protelomerase TelK complexed with substrate DNA

PDB ID 2v6e

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2v6e"
Personal tools