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6mib

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==Crystal structure of the ILK ATP-binding deficient mutant (L207W)/alpha-parvin core complex==
==Crystal structure of the ILK ATP-binding deficient mutant (L207W)/alpha-parvin core complex==
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<StructureSection load='6mib' size='340' side='right' caption='[[6mib]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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<StructureSection load='6mib' size='340' side='right'caption='[[6mib]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6mib]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MIB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MIB FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6mib]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MIB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MIB FirstGlance]. <br>
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</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mib OCA], [http://pdbe.org/6mib PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mib RCSB], [http://www.ebi.ac.uk/pdbsum/6mib PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mib ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mib OCA], [https://pdbe.org/6mib PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mib RCSB], [https://www.ebi.ac.uk/pdbsum/6mib PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mib ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/ILK_HUMAN ILK_HUMAN]] Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. [[http://www.uniprot.org/uniprot/PARVA_HUMAN PARVA_HUMAN]] Plays a role in sarcomere organization and in smooth muscle cell contraction. Required for normal development of the embryonic cardiovascular system, and for normal septation of the heart outflow tract. Plays a role in sprouting angiogenesis and is required for normal adhesion of vascular smooth muscle cells to endothelial cells during blood vessel development (By similarity). Plays a role in the reorganization of the actin cytoskeleton, formation of lamellipodia and ciliogenesis. Plays a role in the establishement of cell polarity, cell adhesion, cell spreading, and directed cell migration.<ref>PMID:11331308</ref> <ref>PMID:11134073</ref> <ref>PMID:15284246</ref> <ref>PMID:20393563</ref>
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[https://www.uniprot.org/uniprot/ILK_HUMAN ILK_HUMAN] Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Dynamic communication between integrin-containing complexes (focal adhesions, FAs) and actin filaments is critical for regulating cell adhesion. Pseudokinase ILK plays a key role in this process but the underlying mechanism remains highly elusive. Here we show that by recruiting FA adaptors PINCH and Parvin into a heterotrimeric complex (IPP), ILK triggers F-actin filament bundling - a process known to generate force/mechanical signal to promote cytoskeleton reassembly and dynamic cell adhesion. Structural, biochemical, and functional analyses revealed that the F-actin bundling is orchestrated by two previously unrecognized WASP-Homology-2 actin binding motifs within IPP, one from PINCH and the other from Parvin. Strikingly, this process is also sensitized to Mg-ATP bound to the pseudoactive site of ILK and its dysregulation severely impairs stress fibers formation, cell spreading, and migration. These data identify a crucial mechanism for ILK, highlighting its uniqueness as a pseudokinase to transduce non-catalytic signal and regulate cell adhesion.
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Non-catalytic signaling by pseudokinase ILK for regulating cell adhesion.,Vaynberg J, Fukuda K, Lu F, Bialkowska K, Chen Y, Plow EF, Qin J Nat Commun. 2018 Oct 26;9(1):4465. doi: 10.1038/s41467-018-06906-7. PMID:30367047<ref>PMID:30367047</ref>
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==See Also==
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*[[Parvin 3D structures|Parvin 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6mib" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Non-specific serine/threonine protein kinase]]
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[[Category: Homo sapiens]]
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[[Category: Fukuda, K]]
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[[Category: Large Structures]]
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[[Category: Qin, J]]
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[[Category: Fukuda K]]
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[[Category: Cell adhesion]]
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[[Category: Qin J]]
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[[Category: Protein complex]]
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[[Category: Pseudokinase]]
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Current revision

Crystal structure of the ILK ATP-binding deficient mutant (L207W)/alpha-parvin core complex

PDB ID 6mib

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