2v9v

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[[Image:2v9v.jpg|left|200px]]
 
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{{Structure
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==Crystal Structure of Moorella thermoacetica SelB(377-511)==
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|PDB= 2v9v |SIZE=350|CAPTION= <scene name='initialview01'>2v9v</scene>, resolution 1.10&Aring;
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<StructureSection load='2v9v' size='340' side='right'caption='[[2v9v]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
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|SITE= <scene name='pdbsite=AC1:Na+Binding+Site+For+Chain+A'>AC1</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
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<table><tr><td colspan='2'>[[2v9v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V9V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V9V FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v9v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v9v OCA], [https://pdbe.org/2v9v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v9v RCSB], [https://www.ebi.ac.uk/pdbsum/2v9v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v9v ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1lva|1LVA]], [[1wsu|1WSU]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v9v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v9v OCA], [http://www.ebi.ac.uk/pdbsum/2v9v PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2v9v RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/SELB_MOOTH SELB_MOOTH] Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v9/2v9v_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2v9v ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the first two winged-helix motifs of translation elongation factor SelB from Moorella thermoacetica has been determined at 1.1 A resolution. Compared with the previous structure of the two domains in conjunction with winged-helix modules 3 and 4, the first winged-helix domain underwent a substantial conformational change during which the alpha-helical and beta-sheet portions of the element opened up like a shell. This conformational rearrangement was elicited by a change in the orientation of Trp396, leading to the disclosure of a bona fide ligand-binding site in the direct vicinity of Trp396. Additionally, the C-terminal tail of the second domain followed a different path compared with the previous structure. It is conceivable that these conformational switches constitute part of the molecular mechanism that underlies the communication between the N-terminal part of SelB, which binds Sec-tRNA(Sec) and GTP, and the C-terminal part of the protein, which binds selenocysteine-insertion sequences.
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'''CRYSTAL STRUCTURE OF MOORELLA THERMOACETICA SELB(377-511)'''
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Conformational switches in winged-helix domains 1 and 2 of bacterial translation elongation factor SelB.,Ganichkin O, Wahl MC Acta Crystallogr D Biol Crystallogr. 2007 Oct;63(Pt 10):1075-81. Epub 2007, Sep 19. PMID:17881825<ref>PMID:17881825</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2v9v" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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The crystal structure of the first two winged-helix motifs of translation elongation factor SelB from Moorella thermoacetica has been determined at 1.1 A resolution. Compared with the previous structure of the two domains in conjunction with winged-helix modules 3 and 4, the first winged-helix domain underwent a substantial conformational change during which the alpha-helical and beta-sheet portions of the element opened up like a shell. This conformational rearrangement was elicited by a change in the orientation of Trp396, leading to the disclosure of a bona fide ligand-binding site in the direct vicinity of Trp396. Additionally, the C-terminal tail of the second domain followed a different path compared with the previous structure. It is conceivable that these conformational switches constitute part of the molecular mechanism that underlies the communication between the N-terminal part of SelB, which binds Sec-tRNA(Sec) and GTP, and the C-terminal part of the protein, which binds selenocysteine-insertion sequences.
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*[[Elongation factor 3D structures|Elongation factor 3D structures]]
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*[[SelB|SelB]]
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==About this Structure==
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== References ==
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2V9V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V9V OCA].
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<references/>
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__TOC__
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==Reference==
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</StructureSection>
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Conformational switches in winged-helix domains 1 and 2 of bacterial translation elongation factor SelB., Ganichkin O, Wahl MC, Acta Crystallogr D Biol Crystallogr. 2007 Oct;63(Pt 10):1075-81. Epub 2007, Sep 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17881825 17881825]
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[[Category: Large Structures]]
[[Category: Moorella thermoacetica]]
[[Category: Moorella thermoacetica]]
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[[Category: Single protein]]
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[[Category: Ganichkin O]]
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[[Category: Ganichkin, O.]]
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[[Category: Wahl MC]]
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[[Category: Wahl, M C.]]
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[[Category: cytoplasm]]
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[[Category: gtp-binding]]
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[[Category: nucleotide-binding]]
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[[Category: protein biosynthesis]]
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[[Category: protein conformational change]]
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[[Category: protein dynamic]]
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[[Category: selenocysteine]]
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[[Category: selenoprotein biosynthesis]]
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[[Category: transcription elongation factor selb]]
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[[Category: winged-helix domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:10:05 2008''
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Current revision

Crystal Structure of Moorella thermoacetica SelB(377-511)

PDB ID 2v9v

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