3bx3

<StructureSection load='3bx3' size='340' side='right' caption='[[3bx3]], [[Resolution|resolution]] 3.00&Aring;' scene=''>

<table><tr><td colspan='2'>[[3bx3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BX3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BX3 FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1m6w|1m6w]], [[1m8x|1m8x]], [[1m8y|1m8y]], [[1m8z|1m8z]], [[1ib2|1ib2]], [[3bwt|3bwt]], [[3bx2|3bx2]]</td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PUF4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bx3 OCA], [http://pdbe.org/3bx3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bx3 RCSB], [http://www.ebi.ac.uk/pdbsum/3bx3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bx3 ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/PUF4_YEAST PUF4_YEAST]] Is not essential for haploid growth, but may affect diploid formation.

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== Publication Abstract from PubMed ==

Pumilio/FBF (PUF) family proteins are found in eukaryotic organisms and regulate gene expression post-transcriptionally by binding to sequences in the 3' untranslated region of target transcripts. PUF proteins contain an RNA binding domain that typically comprises eight alpha-helical repeats, each of which recognizes one RNA base. Some PUF proteins, including yeast Puf4p, have altered RNA binding specificity and use their eight repeats to bind to RNA sequences with nine or ten bases. Here we report the crystal structures of Puf4p alone and in complex with a 9-nucleotide (nt) target RNA sequence, revealing that Puf4p accommodates an 'extra' nucleotide by modest adaptations allowing one base to be turned away from the RNA binding surface. Using structural information and sequence comparisons, we created a mutant Puf4p protein that preferentially binds to an 8-nt target RNA sequence over a 9-nt sequence and restores binding of each protein repeat to one RNA base.

Basis of altered RNA-binding specificity by PUF proteins revealed by crystal structures of yeast Puf4p.,Miller MT, Higgin JJ, Hall TM Nat Struct Mol Biol. 2008 Apr;15(4):397-402. Epub 2008 Mar 9. PMID:18327269<ref>PMID:18327269</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 3bx3" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Atcc 18824]]

[[Category: Hall, T M.T]]

[[Category: Higgin, J J]]

[[Category: Miller, M T]]

[[Category: Ho endonuclease]]

[[Category: Transcription]]