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| | ==X-ray structure of the aquaglyceroporin from Plasmodium falciparum== | | ==X-ray structure of the aquaglyceroporin from Plasmodium falciparum== |
| - | <StructureSection load='3c02' size='340' side='right' caption='[[3c02]], [[Resolution|resolution]] 2.05Å' scene=''> | + | <StructureSection load='3c02' size='340' side='right'caption='[[3c02]], [[Resolution|resolution]] 2.05Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3c02]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C02 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3c02]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C02 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C02 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1fx8|1fx8]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AQP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 Plasmodium falciparum])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c02 OCA], [https://pdbe.org/3c02 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c02 RCSB], [https://www.ebi.ac.uk/pdbsum/3c02 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c02 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c02 OCA], [http://pdbe.org/3c02 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3c02 RCSB], [http://www.ebi.ac.uk/pdbsum/3c02 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3c02 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8WPZ6_PLAFA Q8WPZ6_PLAFA] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Aquaporin|Aquaporin]] | + | *[[Aquaporin 3D structures|Aquaporin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Plasmodium falciparum]] | | [[Category: Plasmodium falciparum]] |
| - | [[Category: CSMP, Center for Structures of Membrane Proteins]]
| + | [[Category: Newby ZE]] |
| - | [[Category: Newby, Z E]] | + | [[Category: Stroud RM]] |
| - | [[Category: Stroud, R M]] | + | |
| - | [[Category: Center for structures of membrane protein]]
| + | |
| - | [[Category: Csmp]]
| + | |
| - | [[Category: Glycerol]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Porin]]
| + | |
| - | [[Category: PSI, Protein structure initiative]]
| + | |
| - | [[Category: Structural genomic]]
| + | |
| - | [[Category: Transmembrane]]
| + | |
| - | [[Category: Transport]]
| + | |
| - | [[Category: Water]]
| + | |
| Structural highlights
Function
Q8WPZ6_PLAFA
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The 2.05-A resolution structure of the aquaglyceroporin from the malarial parasite Plasmodium falciparum (PfAQP), a protein important in the parasite's life cycle, has been solved. The structure provides key evidence for the basis of water versus glycerol selectivity in aquaporin family members. Unlike its closest homolog of known structure, GlpF, the channel conducts both glycerol and water at high rates, framing the question of what determines high water conductance in aquaporin channels. The universally conserved arginine in the selectivity filter is constrained by only two hydrogen bonds in GlpF, whereas there are three in all water-selective aquaporins and in PfAQP. The decreased cost of dehydrating the triply-satisfied arginine cation may provide the basis for high water conductance. The two Asn-Pro-Ala (NPA) regions of PfAQP, which bear rare substitutions to Asn-Leu-Ala (NLA) and Asn-Pro-Ser (NPS), participate in preserving the orientation of the selectivity filter asparagines in the center of the channel.
Crystal structure of the aquaglyceroporin PfAQP from the malarial parasite Plasmodium falciparum.,Newby ZE, O'Connell J 3rd, Robles-Colmenares Y, Khademi S, Miercke LJ, Stroud RM Nat Struct Mol Biol. 2008 Jun;15(6):619-25. Epub 2008 May 25. PMID:18500352[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Newby ZE, O'Connell J 3rd, Robles-Colmenares Y, Khademi S, Miercke LJ, Stroud RM. Crystal structure of the aquaglyceroporin PfAQP from the malarial parasite Plasmodium falciparum. Nat Struct Mol Biol. 2008 Jun;15(6):619-25. Epub 2008 May 25. PMID:18500352 doi:10.1038/nsmb.1431
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