6i38
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of nv2Pizza6-AYW, a circularly permuted designer protein== | |
| + | <StructureSection load='6i38' size='340' side='right'caption='[[6i38]], [[Resolution|resolution]] 1.58Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6i38]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I38 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6I38 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.58Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6i38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i38 OCA], [https://pdbe.org/6i38 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6i38 RCSB], [https://www.ebi.ac.uk/pdbsum/6i38 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6i38 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The beta-propeller fold is adopted by a sequentially diverse family of repeat proteins with apparent rotational symmetry. While the structure is mostly stabilized by hydrophobic interactions, an additional stabilization is provided by hydrogen bonds between the N-and C-termini, which are almost invariably part of the same beta-sheet. This feature is often referred to as the "Velcro" closure. The positioning of the termini within a blade is variable and depends on the protein family. In order to investigate the influence of this location on protein structure, folding and stability, we created different circular permutants, and a circularized version, of the designer propeller protein named Pizza. This protein is perfectly symmetrical, possessing six identical repeats. While all mutants adopt the same structure, the proteins lacking the "Velcro" closure were found to be significantly less resistant to thermal and chemical denaturation. This could explain why such proteins are rarely observed in nature. Interestingly the most common "Velcro" configuration for this protein family was not the most stable among the Pizza variants tested. The circularized version shows dramatically improved stability, which could have implications for future applications. | ||
| - | + | Influence of circular permutations on the structure and stability of a six-fold circular symmetric designer protein.,Mylemans B, Noguchi H, Deridder E, Lescrinier E, Tame JRH, Voet ARD Protein Sci. 2020 Oct 2. doi: 10.1002/pro.3961. PMID:33006397<ref>PMID:33006397</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6i38" style="background-color:#fffaf0;"></div> |
| - | [[Category: Mylemans | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Deridder E]] | ||
| + | [[Category: Mylemans B]] | ||
| + | [[Category: Noguchi H]] | ||
| + | [[Category: Voet ARD]] | ||
Current revision
Crystal structure of nv2Pizza6-AYW, a circularly permuted designer protein
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