6mzc

From Proteopedia

(Difference between revisions)

Current revision

Human TFIID BC core

6mzc, resolution 4.50Å

Structural highlights

6mzc is a 10 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 4.5Å
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

TAF2_HUMAN Microcephaly-thin corpus callosum-intellectual disability syndrome. The disease is caused by mutations affecting the gene represented in this entry.

Function

TAF2_HUMAN Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It requires core promoter-specific cofactors for productive transcription stimulation. TAF2 stabilizes TFIID binding to core promoter.^[1] ^[2]

Publication Abstract from PubMed

The general transcription factor IID (TFIID) is a critical component of the eukaryotic transcription preinitiation complex (PIC) and is responsible for recognizing the core promoter DNA and initiating PIC assembly. We used cryo-electron microscopy (cryo-EM), chemical crosslinking-mass spectrometry (CX-MS) and biochemical reconstitution to determine the complete molecular architecture of TFIID and define the conformational landscape of TFIID in the process of TATA-box binding protein (TBP) loading onto promoter DNA. Our structural analysis revealed five structural states of TFIID in the presence of TFIIA and promoter DNA, showing that the initial binding of TFIID to the downstream promoter positions the upstream DNA and facilitates scanning of TBP for a TATA-box and the subsequent engagement of the promoter. Our findings provide a mechanistic model for the specific loading of TBP by TFIID onto the promoter.

Structure of human TFIID and mechanism of TBP loading onto promoter DNA.,Patel AB, Louder RK, Greber BJ, Grunberg S, Luo J, Fang J, Liu Y, Ranish J, Hahn S, Nogales E Science. 2018 Nov 15. pii: science.aau8872. doi: 10.1126/science.aau8872. PMID:30442764^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kaufmann J, Ahrens K, Koop R, Smale ST, Muller R. CIF150, a human cofactor for transcription factor IID-dependent initiator function. Mol Cell Biol. 1998 Jan;18(1):233-9. PMID:9418870
↑ Martinez E, Ge H, Tao Y, Yuan CX, Palhan V, Roeder RG. Novel cofactors and TFIIA mediate functional core promoter selectivity by the human TAFII150-containing TFIID complex. Mol Cell Biol. 1998 Nov;18(11):6571-83. PMID:9774672
↑ Patel AB, Louder RK, Greber BJ, Grunberg S, Luo J, Fang J, Liu Y, Ranish J, Hahn S, Nogales E. Structure of human TFIID and mechanism of TBP loading onto promoter DNA. Science. 2018 Nov 15. pii: science.aau8872. doi: 10.1126/science.aau8872. PMID:30442764 doi:http://dx.doi.org/10.1126/science.aau8872

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6mzc"

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 6mzc is ON HOLD
+==Human TFIID BC core==
+<SX load='6mzc' size='340' side='right' viewer='molstar' caption='[[6mzc]], [[Resolution|resolution]] 4.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6mzc]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MZC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MZC FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.5&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mzc OCA], [https://pdbe.org/6mzc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mzc RCSB], [https://www.ebi.ac.uk/pdbsum/6mzc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mzc ProSAT]</span></td></tr>
+</table>
+== Disease ==
+[https://www.uniprot.org/uniprot/TAF2_HUMAN TAF2_HUMAN] Microcephaly-thin corpus callosum-intellectual disability syndrome. The disease is caused by mutations affecting the gene represented in this entry.
+== Function ==
+[https://www.uniprot.org/uniprot/TAF2_HUMAN TAF2_HUMAN] Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It requires core promoter-specific cofactors for productive transcription stimulation. TAF2 stabilizes TFIID binding to core promoter.<ref>PMID:9418870</ref> <ref>PMID:9774672</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The general transcription factor IID (TFIID) is a critical component of the eukaryotic transcription preinitiation complex (PIC) and is responsible for recognizing the core promoter DNA and initiating PIC assembly. We used cryo-electron microscopy (cryo-EM), chemical crosslinking-mass spectrometry (CX-MS) and biochemical reconstitution to determine the complete molecular architecture of TFIID and define the conformational landscape of TFIID in the process of TATA-box binding protein (TBP) loading onto promoter DNA. Our structural analysis revealed five structural states of TFIID in the presence of TFIIA and promoter DNA, showing that the initial binding of TFIID to the downstream promoter positions the upstream DNA and facilitates scanning of TBP for a TATA-box and the subsequent engagement of the promoter. Our findings provide a mechanistic model for the specific loading of TBP by TFIID onto the promoter.
-Authors:
+Structure of human TFIID and mechanism of TBP loading onto promoter DNA.,Patel AB, Louder RK, Greber BJ, Grunberg S, Luo J, Fang J, Liu Y, Ranish J, Hahn S, Nogales E Science. 2018 Nov 15. pii: science.aau8872. doi: 10.1126/science.aau8872. PMID:30442764<ref>PMID:30442764</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6mzc" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Transcription initiation factors 3D structures|Transcription initiation factors 3D structures]]
+== References ==
+<references/>
+__TOC__
+</SX>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Fang J]]
+[[Category: Greber BJ]]
+[[Category: Grunberg S]]
+[[Category: Hahn S]]
+[[Category: Liu Y]]
+[[Category: Louder RK]]
+[[Category: Luo J]]
+[[Category: Nogales E]]
+[[Category: Patel AB]]
+[[Category: Ranish J]]

6mzc

From Proteopedia

Current revision

Human TFIID BC core

Structural highlights

Disease

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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