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| - | [[Image:2vkx.jpg|left|200px]] | |
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| - | {{Structure
| + | ==Human NCAM, FN3 domains 1 and 2, M610R mutant== |
| - | |PDB= 2vkx |SIZE=350|CAPTION= <scene name='initialview01'>2vkx</scene>, resolution 2.7Å
| + | <StructureSection load='2vkx' size='340' side='right'caption='[[2vkx]], [[Resolution|resolution]] 2.70Å' scene=''> |
| - | |SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Chain+C'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Chain+C'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Chain+F'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Chain+F'>AC5</scene>, <scene name='pdbsite=AC6:So4+Binding+Site+For+Chain+A'>AC6</scene>, <scene name='pdbsite=AC7:So4+Binding+Site+For+Chain+F'>AC7</scene>, <scene name='pdbsite=AC8:So4+Binding+Site+For+Chain+E'>AC8</scene>, <scene name='pdbsite=AC9:So4+Binding+Site+For+Chain+D'>AC9</scene>, <scene name='pdbsite=BC1:So4+Binding+Site+For+Chain+F'>BC1</scene> and <scene name='pdbsite=BC2:So4+Binding+Site+For+Chain+F'>BC2</scene>
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
| + | <table><tr><td colspan='2'>[[2vkx]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VKX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VKX FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vkx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vkx OCA], [https://pdbe.org/2vkx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vkx RCSB], [https://www.ebi.ac.uk/pdbsum/2vkx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vkx ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=[[2vkw|2VKW]]
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vkx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vkx OCA], [http://www.ebi.ac.uk/pdbsum/2vkx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2vkx RCSB]</span>
| + | == Function == |
| - | }}
| + | [https://www.uniprot.org/uniprot/NCAM1_HUMAN NCAM1_HUMAN] This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. |
| - | | + | == Evolutionary Conservation == |
| - | '''HUMAN NCAM, FN3 DOMAINS 1 AND 2, M610R MUTANT'''
| + | [[Image:Consurf_key_small.gif|200px|right]] |
| - | | + | Check<jmol> |
| - | | + | <jmolCheckbox> |
| - | ==Overview== | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vk/2vkx_consurf.spt"</scriptWhenChecked> |
| - | Activation of the fibroblast growth factor receptor (FGFR) by neural cell adhesion molecule (NCAM) is essential for NCAM-mediated neurite outgrowth. Previous peptide studies have identified two regions in the fibronectin type 3 (FN3)-like domains of NCAM as being important for these activities. Here we report the crystal structure of the NCAM FN3 domain tandem, which reveals an acutely bent domain arrangement. Mutation of a non-conserved surface residue (M610R) led to a second crystal form showing a substantially different conformation. Thus, the FN3 domain linker is highly flexible, suggesting that it corresponds to the hinge seen in electron micrographs of NCAM. The two putative FGFR1-binding segments, one in each NCAM FN3 domain, are situated close to the domain interface. They form a contiguous patch in the more severely bent conformation but become separated upon straightening of the FN3 tandem, suggesting that conformational changes within NCAM may modulate FGFR1 activation. Surface plasmon resonance experiments demonstrated only a very weak interaction between the NCAM FN3 tandem and soluble FGFR1 proteins expressed in mammalian cells (dissociation constant >100 muM). Thus, the NCAM-FGFR1 interaction at the cell surface is likely to depend upon avidity effects due to receptor clustering.
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| - | | + | <text>to colour the structure by Evolutionary Conservation</text> |
| - | ==About this Structure== | + | </jmolCheckbox> |
| - | 2VKX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VKX OCA].
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vkx ConSurf]. |
| - | | + | <div style="clear:both"></div> |
| - | ==Reference== | + | __TOC__ |
| - | Structure of the Tandem Fibronectin Type 3 Domains of Neural Cell Adhesion Molecule., Carafoli F, Saffell JL, Hohenester E, J Mol Biol. 2008 Jan 17;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18261743 18261743]
| + | </StructureSection> |
| | [[Category: Homo sapiens]] | | [[Category: Homo sapiens]] |
| - | [[Category: Single protein]] | + | [[Category: Large Structures]] |
| - | [[Category: Carafoli, F.]] | + | [[Category: Carafoli F]] |
| - | [[Category: Hohenester, E.]] | + | [[Category: Hohenester E]] |
| - | [[Category: Saffell, J L.]] | + | [[Category: Saffell JL]] |
| - | [[Category: adhesion receptor]]
| + | |
| - | [[Category: cell adhesion]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:13:23 2008''
| + | |
