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| | ==Glomerella cingulata apo cutinase== | | ==Glomerella cingulata apo cutinase== |
| - | <StructureSection load='3dcn' size='340' side='right' caption='[[3dcn]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='3dcn' size='340' side='right'caption='[[3dcn]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3dcn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DCN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DCN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3dcn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Colletotrichum_gloeosporioides Colletotrichum gloeosporioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DCN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DCN FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3dd5|3dd5]], [[3dea|3dea]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cutinase Cutinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.74 3.1.1.74] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dcn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dcn OCA], [https://pdbe.org/3dcn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dcn RCSB], [https://www.ebi.ac.uk/pdbsum/3dcn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dcn ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dcn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dcn OCA], [http://pdbe.org/3dcn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3dcn RCSB], [http://www.ebi.ac.uk/pdbsum/3dcn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3dcn ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CUTI1_COLGL CUTI1_COLGL]] Catalyzes the hydrolysis of cutin, a polyester that forms the structure of plant cuticle. Allows pathogenic fungi to penetrate through the cuticular barrier into the host plant during the initial stage of the fungal infection.<ref>PMID:17043825</ref> | + | [https://www.uniprot.org/uniprot/CUTI1_COLGL CUTI1_COLGL] Catalyzes the hydrolysis of cutin, a polyester that forms the structure of plant cuticle. Allows pathogenic fungi to penetrate through the cuticular barrier into the host plant during the initial stage of the fungal infection.<ref>PMID:17043825</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Cutinase|Cutinase]] | + | *[[Cutinase 3D structures|Cutinase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cutinase]] | + | [[Category: Colletotrichum gloeosporioides]] |
| - | [[Category: Berrisford, J M]] | + | [[Category: Large Structures]] |
| - | [[Category: Craven, C J]] | + | [[Category: Berrisford JM]] |
| - | [[Category: Diba, A B.Farah]] | + | [[Category: Craven CJ]] |
| - | [[Category: Hounslow, A M]] | + | [[Category: Farah Diba AB]] |
| - | [[Category: Huang, H]] | + | [[Category: Hounslow AM]] |
| - | [[Category: Mahadi, N M]] | + | [[Category: Huang H]] |
| - | [[Category: Moir, A J.G]] | + | [[Category: Mahadi NM]] |
| - | [[Category: Nathan, S]] | + | [[Category: Moir AJG]] |
| - | [[Category: Nyon, M P]] | + | [[Category: Nathan S]] |
| - | [[Category: Rice, D W]] | + | [[Category: Nyon MP]] |
| - | [[Category: Catalytic triad]]
| + | [[Category: Rice DW]] |
| - | [[Category: Glomerella cingulata]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Secreted]]
| + | |
| - | [[Category: Serine esterase]]
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| Structural highlights
Function
CUTI1_COLGL Catalyzes the hydrolysis of cutin, a polyester that forms the structure of plant cuticle. Allows pathogenic fungi to penetrate through the cuticular barrier into the host plant during the initial stage of the fungal infection.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Cutinase belongs to a group of enzymes that catalyze the hydrolysis of esters and triglycerides. Structural studies on the enzyme from Fusarium solani have revealed the presence of a classic catalytic triad that has been implicated in the enzyme's mechanism. We have solved the crystal structure of Glomerella cingulata cutinase in the absence and in the presence of the inhibitors E600 (diethyl p-nitrophenyl phosphate) and PETFP (3-phenethylthio-1,1,1-trifluoropropan-2-one) to resolutions between 2.6 and 1.9 A. Analysis of these structures reveals that the catalytic triad (Ser136, Asp191, and His204) adopts an unusual configuration with the putative essential histidine His204 swung out of the active site into a position where it is unable to participate in catalysis, with the imidazole ring 11 A away from its expected position. Solution-state NMR experiments are consistent with the disrupted configuration of the triad observed crystallographically. H204N, a site-directed mutant, was shown to be catalytically inactive, confirming the importance of this residue in the enzyme mechanism. These findings suggest that, during its catalytic cycle, cutinase undergoes a significant conformational rearrangement converting the loop bearing the histidine from an inactive conformation, in which the histidine of the triad is solvent exposed, to an active conformation, in which the triad assumes a classic configuration.
Catalysis by Glomerella cingulata Cutinase Requires Conformational Cycling between the Active and Inactive States of Its Catalytic Triad.,Nyon MP, Rice DW, Berrisford JM, Hounslow AM, Moir AJ, Huang H, Nathan S, Mahadi NM, Bakar FD, Craven CJ J Mol Biol. 2008 Oct 31. PMID:18983850[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Chen Z, Franco CF, Baptista RP, Cabral JM, Coelho AV, Rodrigues CJ Jr, Melo EP. Purification and identification of cutinases from Colletotrichum kahawae and Colletotrichum gloeosporioides. Appl Microbiol Biotechnol. 2007 Jan;73(6):1306-13. Epub 2006 Oct 17. PMID:17043825 doi:http://dx.doi.org/10.1007/s00253-006-0605-1
- ↑ Nyon MP, Rice DW, Berrisford JM, Hounslow AM, Moir AJ, Huang H, Nathan S, Mahadi NM, Bakar FD, Craven CJ. Catalysis by Glomerella cingulata Cutinase Requires Conformational Cycling between the Active and Inactive States of Its Catalytic Triad. J Mol Biol. 2008 Oct 31. PMID:18983850 doi:S0022-2836(08)01341-7
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