|
|
| (One intermediate revision not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal structure of Cj0915, a hexameric hotdog fold thioesterase of Campylobacter jejuni== | | ==Crystal structure of Cj0915, a hexameric hotdog fold thioesterase of Campylobacter jejuni== |
| - | <StructureSection load='3d6l' size='340' side='right' caption='[[3d6l]], [[Resolution|resolution]] 2.59Å' scene=''> | + | <StructureSection load='3d6l' size='340' side='right'caption='[[3d6l]], [[Resolution|resolution]] 2.59Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3d6l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp. jejuni" smibert 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D6L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3D6L FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3d6l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D6L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3D6L FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.59Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Cj0915 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197 "Campylobacter fetus subsp. jejuni" Smibert 1974])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3d6l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d6l OCA], [http://pdbe.org/3d6l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3d6l RCSB], [http://www.ebi.ac.uk/pdbsum/3d6l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3d6l ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3d6l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d6l OCA], [https://pdbe.org/3d6l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3d6l RCSB], [https://www.ebi.ac.uk/pdbsum/3d6l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3d6l ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q0P9Y4_CAMJE Q0P9Y4_CAMJE] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| Line 31: |
Line 33: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Campylobacter fetus subsp. jejuni smibert 1974]] | |
| - | [[Category: Yeo, H J]] | |
| - | [[Category: Yokoyama, T]] | |
| - | [[Category: Acyl-coa]] | |
| | [[Category: Campylobacter jejuni]] | | [[Category: Campylobacter jejuni]] |
| - | [[Category: Hot dog fold]] | + | [[Category: Large Structures]] |
| - | [[Category: Hydrolase]] | + | [[Category: Yeo HJ]] |
| - | [[Category: Thioesterase]] | + | [[Category: Yokoyama T]] |
| Structural highlights
Function
Q0P9Y4_CAMJE
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Acyl-coenzyme A (CoA) thioesterases are a large family of enzymes that hydrolyze acyl-CoA esters to the free fatty acid and CoA and thereby regulate essential cellular functions such as lipid metabolism, membrane synthesis, signal transduction, and gene transcription. To better understand the virulence mechanisms of Campylobacter jejuni, and its possible link to membrane lipid biosynthesis, we have investigated C. jejuni thioesterases, annotated as putative proteins. While little is known about fatty acid biosynthesis and regulation in C. jejuni, remarkable differences in the genome and its organization from Escherichia coli, the paradigm system, raise questions as to the functions of these putative proteins. Here we present the crystal structure and biochemical analysis of Cj0915, defining the first functional thioesterase from C. jejuni. The structure of Cj0915 reveals the hot dog fold with an YciA-type hexameric assembly. Enzymatic assays performed with the purified protein show that Cj0915 is an efficient thioesterase with a broad specificity toward acyl-CoA substrates. This study provides a framework for investigation on roles of the Cj0915 thioesterase in virulence, and functional activities associated with the Cj0915 thioesterase in vivo.
Structure and function of a Campylobacter jejuni thioesterase Cj0915, a hexameric hot dog fold enzyme.,Yokoyama T, Choi KJ, Bosch AM, Yeo HJ Biochim Biophys Acta. 2009 Jul;1794(7):1073-81. Epub 2009 Mar 17. PMID:19303060[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yokoyama T, Choi KJ, Bosch AM, Yeo HJ. Structure and function of a Campylobacter jejuni thioesterase Cj0915, a hexameric hot dog fold enzyme. Biochim Biophys Acta. 2009 Jul;1794(7):1073-81. Epub 2009 Mar 17. PMID:19303060 doi:10.1016/j.bbapap.2009.03.002
|
