6i5f
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6i5f is ON HOLD Authors: Bhairosing-Kok, D., Groothuizen, F.S., Fish, A., Dharadhar, S., Winterwerp, H.H.K., Sixma, T.K. Description: Crystal struc...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of DNA-free E.coli MutS P839E dimer mutant== | |
| + | <StructureSection load='6i5f' size='340' side='right'caption='[[6i5f]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6i5f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I5F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6I5F FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6i5f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i5f OCA], [https://pdbe.org/6i5f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6i5f RCSB], [https://www.ebi.ac.uk/pdbsum/6i5f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6i5f ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/MUTS_ECOLI MUTS_ECOLI] This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | DNA mismatch repair (MMR) corrects mismatches, small insertions and deletions in DNA during DNA replication. While scanning for mismatches, dimers of MutS embrace the DNA helix with their lever and clamp domains. Previous studies indicated generic flexibility of the lever and clamp domains of MutS prior to DNA binding, but whether this was important for MutS function was unknown. Here, we present a novel crystal structure of DNA-free Escherichia coli MutS. In this apo-structure, the clamp domains are repositioned due to kinking at specific sites in the coiled-coil region in the lever domains, suggesting a defined hinge point. We made mutations at the coiled-coil hinge point. The mutants made to disrupt the helical fold at the kink site diminish DNA binding, whereas those made to increase stability of coiled-coil result in stronger DNA binding. These data suggest that the site-specific kinking of the coiled-coil in the lever domain is important for loading of this ABC-ATPase on DNA. | ||
| - | + | Sharp kinking of a coiled-coil in MutS allows DNA binding and release.,Bhairosing-Kok D, Groothuizen FS, Fish A, Dharadhar S, Winterwerp HHK, Sixma TK Nucleic Acids Res. 2019 Aug 2. pii: 5542872. doi: 10.1093/nar/gkz649. PMID:31372631<ref>PMID:31372631</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6i5f" style="background-color:#fffaf0;"></div> |
| - | [[Category: Bhairosing-Kok | + | |
| - | [[Category: Fish | + | ==See Also== |
| - | [[Category: | + | *[[DNA mismatch repair protein 3D structures|DNA mismatch repair protein 3D structures]] |
| - | [[Category: Sixma | + | == References == |
| - | [[Category: Winterwerp | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Escherichia coli]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bhairosing-Kok D]] | ||
| + | [[Category: Dharadhar S]] | ||
| + | [[Category: Fish A]] | ||
| + | [[Category: Groothuizen FS]] | ||
| + | [[Category: Sixma TK]] | ||
| + | [[Category: Winterwerp HHK]] | ||
Current revision
Crystal structure of DNA-free E.coli MutS P839E dimer mutant
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