6ikk
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of YfiB(L43P) in complex with YfiR== | |
| + | <StructureSection load='6ikk' size='340' side='right'caption='[[6ikk]], [[Resolution|resolution]] 2.19Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6ikk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IKK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IKK FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.19Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ikk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ikk OCA], [https://pdbe.org/6ikk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ikk RCSB], [https://www.ebi.ac.uk/pdbsum/6ikk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ikk ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/YFIB_PSEAE YFIB_PSEAE] Activates the diguanylate cyclase TpbB/YfiN by sequestering YfiR at the outer membrane, which counteracts the YfiR-mediated repression of TpbB/YfiN at the inner membrane and leads to increased c-di-GMP production (PubMed:20300602, PubMed:22719254). May act as a sensor of envelope stress (PubMed:20300602, PubMed:22719254).<ref>PMID:20300602</ref> <ref>PMID:22719254</ref> Part of the YfiB-TpbB-YfiR (or yfiBNR) system, encoding a tripartite signaling module that modulates intracellular c-di-GMP levels (PubMed:20300602, PubMed:22719254). The system is a key regulator of the small colony variant (SCV) phenotype, and plays an important role in biofilm formation and in vivo persistence (PubMed:20300602). The c-di-GMP produced by TpbB/YfiN stimulates the production of the Pel and Psl exopolysaccharides, which promotes surface attachment, generates an SCV phenotype and confers resistance against phagocytosis (PubMed:20300602).<ref>PMID:20300602</ref> <ref>PMID:22719254</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacterial cyclic-di-GMP (c-di-GMP) is an important messenger molecule that influences diverse cellular processes including motility, virulence and cytotoxicity systems, polysaccharide synthesis and biofilm formation. The YfiBNR tripartite signalling system in P. aeruginosa modulates the cellular c-di-GMP levels in response to signals received from the periplasm. In this study, we analyse the structures of activating mutants of the outer membrane protein YfiB that give rise to increased surface attachment and biofilm formation. The F48S and W55L mutants of YfiB(27-168) crystallize in the same dimeric arrangement as our previously reported YfiB structures that preclude complex formation with YfiR. The L43P mutant of YfiB(27-168) is monomeric and forms a stable complex with YfiR. The YfiB(L43P)-YfiR crystal structure reveals a dramatic rearrangement of the N-terminal fragment, which is implicated in increased YfiB activation and membrane attachment, upon YfiR binding. Comparison with our previous complex structure between YfiB(59-168) and YfiR reveals extensive interactions between the N-terminal fragment of YfiB (residues 35-55) and YfiR. | ||
| - | + | Structural analysis of activating mutants of YfiB from Pseudomonas aeruginosa PAO1.,Li S, Li T, Teng X, Lou X, Xu Y, Zhang Q, Bartlam M Biochem Biophys Res Commun. 2018 Dec 2;506(4):997-1003. doi:, 10.1016/j.bbrc.2018.10.190. Epub 2018 Nov 4. PMID:30404734<ref>PMID:30404734</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6ikk" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas aeruginosa PAO1]] | ||
| + | [[Category: Bartlam M]] | ||
| + | [[Category: Li S]] | ||
| + | [[Category: Zhang Q]] | ||
Current revision
Crystal structure of YfiB(L43P) in complex with YfiR
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