6n01
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of apo AztD from Citrobacter koseri== | |
| + | <StructureSection load='6n01' size='340' side='right'caption='[[6n01]], [[Resolution|resolution]] 1.98Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6n01]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrobacter_koseri_ATCC_BAA-895 Citrobacter koseri ATCC BAA-895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N01 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N01 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.98Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n01 OCA], [https://pdbe.org/6n01 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n01 RCSB], [https://www.ebi.ac.uk/pdbsum/6n01 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n01 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A8AF35_CITK8 A8AF35_CITK8] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Zinc acquisition from limited environments is critical for bacterial survival and pathogenesis. AztD has been identified as a periplasmic or cell surface zinc-binding protein in numerous bacterial species. In Paracoccus denitrificans, AztD can transfer zinc directly to AztC, the solute binding protein for a zinc-specific ATP-binding cassette transporter system, suggesting a role in zinc acquisition and homeostasis. Here, we present the first cry stal structures of AztD from P. denitrificans and tbe human pathogen Citrobacter koseri, revealing a beta-propeller fold and two high-affinity zinc-binding sites that are highly conserved among AztD homologs. These structures combined with transfer assays using WT and mutant proteins provide rare insight into the mechanism of direct zinc transfer from one protein to another. Given the importance of zinc import to bacterial pathogenesis, these insights may prove valuable to the development of zinc transfer inhibitors as antibiotics. | ||
| - | + | Crystal structures of AztD provide mechanistic insights into direct zinc transfer between proteins.,Neupane DP, Fullam SH, Chacon KN, Yukl ET Commun Biol. 2019 Aug 9;2:308. doi: 10.1038/s42003-019-0542-z. eCollection 2019. PMID:31428696<ref>PMID:31428696</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Neupane | + | <div class="pdbe-citations 6n01" style="background-color:#fffaf0;"></div> |
| - | [[Category: Yukl | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Citrobacter koseri ATCC BAA-895]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Neupane DP]] | ||
| + | [[Category: Yukl ET]] | ||
Current revision
Structure of apo AztD from Citrobacter koseri
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