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| | ==Crystal structure of CRISPR associated protein== | | ==Crystal structure of CRISPR associated protein== |
| - | <StructureSection load='3s5u' size='340' side='right' caption='[[3s5u]], [[Resolution|resolution]] 2.70Å' scene=''> | + | <StructureSection load='3s5u' size='340' side='right'caption='[[3s5u]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3s5u]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis_atcc_4200 Enterococcus faecalis atcc 4200]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S5U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S5U FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3s5u]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis_ATCC_4200 Enterococcus faecalis ATCC 4200]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S5U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S5U FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EFDG_01320 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=565642 Enterococcus faecalis ATCC 4200])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s5u OCA], [http://pdbe.org/3s5u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3s5u RCSB], [http://www.ebi.ac.uk/pdbsum/3s5u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3s5u ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s5u OCA], [https://pdbe.org/3s5u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s5u RCSB], [https://www.ebi.ac.uk/pdbsum/3s5u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s5u ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CSN2_ENTFL CSN2_ENTFL]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). Binds dsDNA, binding is disrupted by EGTA. | + | [https://www.uniprot.org/uniprot/CSN2_ENTFL CSN2_ENTFL] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). Binds dsDNA, binding is disrupted by EGTA. |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated protein genes (cas genes) are widespread in bacteria and archaea. They form a line of RNA-based immunity to eradicate invading bacteriophages and malicious plasmids. A key molecular event during this process is the acquisition of new spacers into the CRISPR loci to guide the selective degradation of the matching foreign genetic elements. Csn2 is a Nmeni subtype-specific cas gene required for new spacer acquisition. Here we characterize the Enterococcus faecalis Csn2 protein as a double-stranded (ds-) DNA-binding protein and report its 2.7 A tetrameric ring structure. The inner circle of the Csn2 tetrameric ring is approximately 26 A wide and populated with conserved lysine residues poised for nonspecific interactions with ds-DNA. Each Csn2 protomer contains an alpha/beta domain and an alpha-helical domain; significant hinge motion was observed between these two domains. Ca(2+) was located at strategic positions in the oligomerization interface. We further showed that removal of Ca(2+) ions altered the oligomerization state of Csn2, which in turn severely decreased its affinity for ds-DNA. In summary, our results provided the first insight into the function of the Csn2 protein in CRISPR adaptation by revealing that it is a ds-DNA-binding protein functioning at the quaternary structure level and regulated by Ca(2+) ions.
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| - | Crystal structure of clustered regularly interspaced short palindromic repeats (CRISPR)-associated Csn2 protein revealed Ca2+-dependent double-stranded DNA binding activity.,Nam KH, Kurinov I, Ke A J Biol Chem. 2011 Sep 2;286(35):30759-68. doi: 10.1074/jbc.M111.256263. Epub 2011, Jun 21. PMID:21697083<ref>PMID:21697083</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 3s5u" style="background-color:#fffaf0;"></div>
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| - | == References ==
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| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Enterococcus faecalis atcc 4200]] | + | [[Category: Enterococcus faecalis ATCC 4200]] |
| - | [[Category: Ke, A]] | + | [[Category: Large Structures]] |
| - | [[Category: Nam, K H]] | + | [[Category: Ke A]] |
| - | [[Category: Crispr]] | + | [[Category: Nam KH]] |
| - | [[Category: Crispr adaptation mechanism]]
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| - | [[Category: Dna binding protein]]
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| - | [[Category: Dsdna binding]]
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| - | [[Category: New spacer aquisition]]
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