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| | ==Voltage-dependent K+ channel beta subunit (W121A) in complex with cortisone== | | ==Voltage-dependent K+ channel beta subunit (W121A) in complex with cortisone== |
| - | <StructureSection load='3eb3' size='340' side='right' caption='[[3eb3]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='3eb3' size='340' side='right'caption='[[3eb3]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3eb3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EB3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EB3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3eb3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EB3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EB3 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PDN:17,21-DIHYDROXYPREGNA-1,4-DIENE-3,11,20-TRIONE'>PDN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1qrq|1qrq]], [[1exb|1exb]], [[3eau|3eau]], [[3eb4|3eb4]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PDN:17,21-DIHYDROXYPREGNA-1,4-DIENE-3,11,20-TRIONE'>PDN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Kcnab2, Ckbeta2, Kcnb3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eb3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eb3 OCA], [https://pdbe.org/3eb3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eb3 RCSB], [https://www.ebi.ac.uk/pdbsum/3eb3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eb3 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eb3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eb3 OCA], [http://pdbe.org/3eb3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3eb3 RCSB], [http://www.ebi.ac.uk/pdbsum/3eb3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3eb3 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/KCAB2_RAT KCAB2_RAT]] Accessory potassium channel protein which modulates the activity of the pore-forming alpha subunit. | + | [https://www.uniprot.org/uniprot/KCAB2_RAT KCAB2_RAT] Accessory potassium channel protein which modulates the activity of the pore-forming alpha subunit. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Potassium Channel|Potassium Channel]] | + | *[[Potassium channel 3D structures|Potassium channel 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Buffalo rat]] | + | [[Category: Large Structures]] |
| - | [[Category: Bhosle, R C]] | + | [[Category: Rattus norvegicus]] |
| - | [[Category: Cao, Y]] | + | [[Category: Bhosle RC]] |
| - | [[Category: Kabaleeswaran, V]] | + | [[Category: Cao Y]] |
| - | [[Category: Li, H]] | + | [[Category: Kabaleeswaran V]] |
| - | [[Category: Pan, Y]] | + | [[Category: Li H]] |
| - | [[Category: Weng, J]] | + | [[Category: Pan Y]] |
| - | [[Category: Zhou, M]] | + | [[Category: Weng J]] |
| - | [[Category: Cortisone]]
| + | [[Category: Zhou M]] |
| - | [[Category: Cytoplasm]]
| + | |
| - | [[Category: Interface]]
| + | |
| - | [[Category: Ion transport]]
| + | |
| - | [[Category: Ionic channel]]
| + | |
| - | [[Category: Kvbeta]]
| + | |
| - | [[Category: Nadp]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Phosphoprotein]]
| + | |
| - | [[Category: Potassium]]
| + | |
| - | [[Category: Potassium transport]]
| + | |
| - | [[Category: Transport]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| - | [[Category: Voltage-gated channel]]
| + | |
| Structural highlights
Function
KCAB2_RAT Accessory potassium channel protein which modulates the activity of the pore-forming alpha subunit.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The Shaker family voltage-dependent potassium channels (Kv1) are expressed in a wide variety of cells and are essential for cellular excitability. In humans, loss-of-function mutations of Kv1 channels lead to hyperexcitability and are directly linked to episodic ataxia and atrial fibrillation. All Kv1 channels assemble with beta subunits (Kv betas), and certain Kv betas, for example Kv beta 1, have an N-terminal segment that closes the channel by the N-type inactivation mechanism. In principle, dissociation of Kv beta 1, although never reported, should eliminate inactivation and thus potentiate Kv1 current. We found that cortisone increases rat Kv1 channel activity by binding to Kv beta 1. A crystal structure of the Kv beta-cortisone complex was solved to 1.82-A resolution and revealed novel cortisone binding sites. Further studies demonstrated that cortisone promotes dissociation of Kv beta. The new mode of channel modulation may be explored by native or synthetic ligands to fine-tune cellular excitability.
Cortisone dissociates the Shaker family K+ channels from their beta subunits.,Pan Y, Weng J, Kabaleeswaran V, Li H, Cao Y, Bhosle RC, Zhou M Nat Chem Biol. 2008 Nov;4(11):708-14. Epub 2008 Sep 21. PMID:18806782[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Pan Y, Weng J, Kabaleeswaran V, Li H, Cao Y, Bhosle RC, Zhou M. Cortisone dissociates the Shaker family K+ channels from their beta subunits. Nat Chem Biol. 2008 Nov;4(11):708-14. Epub 2008 Sep 21. PMID:18806782 doi:10.1038/nchembio.114
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