2z4p

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[[Image:2z4p.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of FFRP-DM1==
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|PDB= 2z4p |SIZE=350|CAPTION= <scene name='initialview01'>2z4p</scene>, resolution 1.95&Aring;
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<StructureSection load='2z4p' size='340' side='right'caption='[[2z4p]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ILE:ISOLEUCINE'>ILE</scene>
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<table><tr><td colspan='2'>[[2z4p]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z4P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z4P FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ILE:ISOLEUCINE'>ILE</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z4p OCA], [https://pdbe.org/2z4p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z4p RCSB], [https://www.ebi.ac.uk/pdbsum/2z4p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z4p ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z4p OCA], [http://www.ebi.ac.uk/pdbsum/2z4p PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2z4p RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/O73983_PYRHO O73983_PYRHO]
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== Evolutionary Conservation ==
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'''Crystal structure of FFRP-DM1'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z4/2z4p_consurf.spt"</scriptWhenChecked>
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Feast/famine regulatory proteins (FFRPs) comprise the largest group of archaeal transcription factors. Crystal structures of an FFRP, DM1 from Pyrococcus, were determined in complex with isoleucine, which increases the association state of DM1 to form octamers, and with selenomethionine, which decreases it to maintain dimers under some conditions. Asp39 and Thr/Ser at 69-71 were identified as being important for interaction with the ligand main chain. By analyzing residues surrounding the ligand side chain, partner ligands were identified for various FFRPs from Pyrococcus, e.g., lysine facilitates homo-octamerization of FL11, and arginine facilitates hetero-octamerization of FL11 and DM1. Transcription of the fl11 gene and lysine synthesis are regulated by shifting the equilibrium between association states of FL11 and by shifting the equilibrium toward association with DM1, in response to amino acid availability. With FFRPs also appearing in eubacteria, the origin of such regulation can be traced back to the common ancestor of all extant organisms, serving as a prototype of transcription regulations, now highly diverged.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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2Z4P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z4P OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z4p ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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A structural code for discriminating between transcription signals revealed by the feast/famine regulatory protein DM1 in complex with ligands., Okamura H, Yokoyama K, Koike H, Yamada M, Shimowasa A, Kabasawa M, Kawashima T, Suzuki M, Structure. 2007 Oct;15(10):1325-38. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17937921 17937921]
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</StructureSection>
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[[Category: Pyrococcus horikoshii]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Pyrococcus horikoshii OT3]]
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[[Category: Koike, H.]]
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[[Category: Koike H]]
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[[Category: Kudo, N.]]
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[[Category: Kudo N]]
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[[Category: Suzuki, M.]]
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[[Category: Suzuki M]]
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[[Category: Yamada, M.]]
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[[Category: Yamada M]]
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[[Category: dna binding protein]]
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[[Category: feast/famine regulatory protein]]
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[[Category: ffrp]]
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[[Category: transcriptional regulator]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:18:32 2008''
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Current revision

Crystal structure of FFRP-DM1

PDB ID 2z4p

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