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| | ==Structure of the alcaligin outer membrane recepteur FauA from Bordetella pertussis== | | ==Structure of the alcaligin outer membrane recepteur FauA from Bordetella pertussis== |
| - | <StructureSection load='3efm' size='340' side='right' caption='[[3efm]], [[Resolution|resolution]] 2.33Å' scene=''> | + | <StructureSection load='3efm' size='340' side='right'caption='[[3efm]], [[Resolution|resolution]] 2.33Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3efm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_tussis-convulsivae"_lehmann_and_neumann_1927 "bacterium tussis-convulsivae" lehmann and neumann 1927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EFM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3efm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bordetella_pertussis Bordetella pertussis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EFM FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.33Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fauA, BP2463 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=520 "Bacterium tussis-convulsivae" Lehmann and Neumann 1927])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3efm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3efm OCA], [http://pdbe.org/3efm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3efm RCSB], [http://www.ebi.ac.uk/pdbsum/3efm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3efm ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3efm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3efm OCA], [https://pdbe.org/3efm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3efm RCSB], [https://www.ebi.ac.uk/pdbsum/3efm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3efm ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9X6A5_BORPT Q9X6A5_BORPT] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacterium tussis-convulsivae lehmann and neumann 1927]] | + | [[Category: Bordetella pertussis]] |
| - | [[Category: Armstrong, S K]] | + | [[Category: Large Structures]] |
| - | [[Category: Brillet, K]] | + | [[Category: Armstrong SK]] |
| - | [[Category: Cobessi, D]] | + | [[Category: Brillet K]] |
| - | [[Category: Lauber, E]] | + | [[Category: Cobessi D]] |
| - | [[Category: Reimmann, C]] | + | [[Category: Lauber E]] |
| - | [[Category: Membrane]]
| + | [[Category: Reimmann C]] |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Membrane receptor]]
| + | |
| - | [[Category: Membrane transporter]]
| + | |
| - | [[Category: Receptor]]
| + | |
| - | [[Category: Siderophore]]
| + | |
| - | [[Category: Tonb box]]
| + | |
| Structural highlights
Function
Q9X6A5_BORPT
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Bordetella pertussis is the bacterial agent of whooping cough in humans. Under iron-limiting conditions, it produces the siderophore alcaligin. Released to the extracellular environment, alcaligin chelates iron, which is then taken up as a ferric alcaligin complex via the FauA outer membrane transporter. FauA belongs to a family of TonB-dependent outer membrane transporters that function using energy derived from the proton motive force. Using an in-house protocol for membrane-protein expression, purification and crystallization, FauA was crystallized in its apo form together with three other TonB-dependent transporters from different organisms. Here, the protocol used to study FauA is described and its three-dimensional structure determined at 2.3 A resolution is discussed.
Use of an in-house approach to study the three-dimensional structures of various outer membrane proteins: structure of the alcaligin outer membrane transporter FauA from Bordetella pertussis.,Brillet K, Meksem A, Lauber E, Reimmann C, Cobessi D Acta Crystallogr D Biol Crystallogr. 2009 Apr;65(Pt 4):326-31. Epub 2009 Mar 19. PMID:19307713[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Brillet K, Meksem A, Lauber E, Reimmann C, Cobessi D. Use of an in-house approach to study the three-dimensional structures of various outer membrane proteins: structure of the alcaligin outer membrane transporter FauA from Bordetella pertussis. Acta Crystallogr D Biol Crystallogr. 2009 Apr;65(Pt 4):326-31. Epub 2009 Mar 19. PMID:19307713 doi:10.1107/S0907444909002200
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