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| | ==the 1:2 complex between a Nterminal elongated prolactin and the extra cellular domain of the rat prolactin receptor== | | ==the 1:2 complex between a Nterminal elongated prolactin and the extra cellular domain of the rat prolactin receptor== |
| - | <StructureSection load='3ew3' size='340' side='right' caption='[[3ew3]], [[Resolution|resolution]] 3.80Å' scene=''> | + | <StructureSection load='3ew3' size='340' side='right'caption='[[3ew3]], [[Resolution|resolution]] 3.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3ew3]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EW3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EW3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3ew3]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EW3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EW3 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PRL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), Prlr ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.8Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ew3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ew3 OCA], [http://pdbe.org/3ew3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ew3 RCSB], [http://www.ebi.ac.uk/pdbsum/3ew3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ew3 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ew3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ew3 OCA], [https://pdbe.org/3ew3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ew3 RCSB], [https://www.ebi.ac.uk/pdbsum/3ew3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ew3 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PRL_HUMAN PRL_HUMAN]] Prolactin acts primarily on the mammary gland by promoting lactation. [[http://www.uniprot.org/uniprot/PRLR_RAT PRLR_RAT]] This is a receptor for the anterior pituitary hormone prolactin. | + | [https://www.uniprot.org/uniprot/PRL_HUMAN PRL_HUMAN] Prolactin acts primarily on the mammary gland by promoting lactation. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | <jmolCheckbox> | | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/3ew3_consurf.spt"</scriptWhenChecked> | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/3ew3_consurf.spt"</scriptWhenChecked> |
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> | | </jmolCheckbox> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Buffalo rat]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Human]] | + | [[Category: Large Structures]] |
| - | [[Category: Broutin, I]] | + | [[Category: Rattus norvegicus]] |
| - | [[Category: England, P]] | + | [[Category: Broutin I]] |
| - | [[Category: Goffin, V]] | + | [[Category: England P]] |
| - | [[Category: Jomain, J B]] | + | [[Category: Goffin V]] |
| - | [[Category: Alternative splicing]]
| + | [[Category: Jomain JB]] |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Glycoprotein]]
| + | |
| - | [[Category: Hormone]]
| + | |
| - | [[Category: Hormone-hormone receptor complex]]
| + | |
| - | [[Category: Lactation]]
| + | |
| - | [[Category: Membrane]]
| + | |
| - | [[Category: Receptor]]
| + | |
| - | [[Category: Secreted]]
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| - | [[Category: Transmembrane]]
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| Structural highlights
Function
PRL_HUMAN Prolactin acts primarily on the mammary gland by promoting lactation.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
We report the first crystal structure of a 1:2 hormone.receptor complex that involves prolactin (PRL) as the ligand, at 3.8-A resolution. Stable ternary complexes were obtained by generating affinity-matured PRL variants harboring an N-terminal tail from ovine placental lactogen, a closely related PRL receptor (PRLR) ligand. This structure allows one to draw up an exhaustive inventory of the residues involved at the PRL.PRLR site 2 interface, consistent with all previously reported site-directed mutagenesis data. We propose, with this description, an interaction model involving three structural components of PRL site 2 ("three-pin plug"): the conserved glycine 129 of helix alpha3, the hydrogen bond network involving surrounding residues (glycine cavity), and the N terminus. The model provides a molecular basis for the properties of the different PRL analogs designed to date, including PRLR antagonists. Finally, comparison of our 1:2 PRL.PRLR(2) structure with those of free PRL and its 1:1 complex indicates that the structure of PRL undergoes significant changes when binding the first, but not the second receptor. This suggests that the second PRLR moiety adapts to the 1:1 complex rather than the opposite. In conclusion, this structure will be a useful guiding tool for further investigations of the molecular mechanisms involved in PRLR dimerization and activation, as well as for the optimization of PRLR antagonists, an emerging class of compounds with high therapeutic potential against breast and prostate cancer.
Crystal structure of an affinity-matured prolactin complexed to its dimerized receptor reveals the topology of hormone binding site 2.,Broutin I, Jomain JB, Tallet E, van Agthoven J, Raynal B, Hoos S, Kragelund BB, Kelly PA, Ducruix A, England P, Goffin V J Biol Chem. 2010 Mar 12;285(11):8422-33. Epub 2010 Jan 6. PMID:20053995[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Broutin I, Jomain JB, Tallet E, van Agthoven J, Raynal B, Hoos S, Kragelund BB, Kelly PA, Ducruix A, England P, Goffin V. Crystal structure of an affinity-matured prolactin complexed to its dimerized receptor reveals the topology of hormone binding site 2. J Biol Chem. 2010 Mar 12;285(11):8422-33. Epub 2010 Jan 6. PMID:20053995 doi:10.1074/jbc.M109.089128
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