6ian
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==T. brucei IFT22/74/81 GTP-bound crystal structure== | |
| + | <StructureSection load='6ian' size='340' side='right'caption='[[6ian]], [[Resolution|resolution]] 3.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6ian]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei_TREU927 Trypanosoma brucei brucei TREU927] and [https://en.wikipedia.org/wiki/Trypanosoma_brucei_equiperdum Trypanosoma brucei equiperdum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IAN FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ian FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ian OCA], [https://pdbe.org/6ian PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ian RCSB], [https://www.ebi.ac.uk/pdbsum/6ian PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ian ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q57WF2_TRYB2 Q57WF2_TRYB2] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Intraflagellar transport (IFT) relies on motor proteins and the IFT complex to construct cilia and flagella. The IFT complex subunit IFT22/RabL5 has sequence similarity with small GTPases although the nucleotide specificity is unclear because of non-conserved G4/G5 motifs. We show that IFT22 specifically associates with G-nucleotides and present crystal structures of IFT22 in complex with GDP, GTP, and with IFT74/81. Our structural analysis unravels an unusual GTP/GDP-binding mode of IFT22 bypassing the classical G4 motif. The GTPase switch regions of IFT22 become ordered upon complex formation with IFT74/81 and mediate most of the IFT22-74/81 interactions. Structure-based mutagenesis reveals that association of IFT22 with the IFT complex is essential for flagellum construction in Trypanosoma brucei although IFT22 GTP-loading is not strictly required. | ||
| - | + | Binding of IFT22 to the intraflagellar transport complex is essential for flagellum assembly.,Wachter S, Jung J, Shafiq S, Basquin J, Fort C, Bastin P, Lorentzen E EMBO J. 2019 Apr 2. pii: embj.2018101251. doi: 10.15252/embj.2018101251. PMID:30940671<ref>PMID:30940671</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6ian" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Basquin | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Trypanosoma brucei brucei TREU927]] | ||
| + | [[Category: Trypanosoma brucei equiperdum]] | ||
| + | [[Category: Basquin J]] | ||
| + | [[Category: Lorentzen E]] | ||
| + | [[Category: Wachter S]] | ||
Current revision
T. brucei IFT22/74/81 GTP-bound crystal structure
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