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2zco

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[[Image:2zco.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of the C(30) carotenoid dehydrosqualene synthase from Staphylococcus aureus==
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|PDB= 2zco |SIZE=350|CAPTION= <scene name='initialview01'>2zco</scene>, resolution 1.58&Aring;
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<StructureSection load='2zco' size='340' side='right'caption='[[2zco]], [[Resolution|resolution]] 1.58&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2zco]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZCO FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.58&#8491;</td></tr>
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|GENE= crtM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zco OCA], [https://pdbe.org/2zco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zco RCSB], [https://www.ebi.ac.uk/pdbsum/2zco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zco ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[2zcp|2ZCP]], [[2zcq|2ZCQ]], [[2zcr|2ZCR]], [[2zcs|2ZCS]]
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zco OCA], [http://www.ebi.ac.uk/pdbsum/2zco PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2zco RCSB]</span>
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[https://www.uniprot.org/uniprot/CRTM_STAAU CRTM_STAAU]
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zc/2zco_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zco ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of the C(30) carotenoid dehydrosqualene synthase from Staphylococcus aureus'''
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==See Also==
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*[[Squalene synthase|Squalene synthase]]
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__TOC__
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==Overview==
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</StructureSection>
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Staphylococcus aureus produces hospital- and community-acquired infections, with methicillin-resistant S. aureus posing a serious public health threat. The golden carotenoid pigment of S. aureus, staphyloxanthin, promotes resistance to reactive oxygen species and host neutrophil-based killing, and early enzymatic steps in staphyloxanthin production resemble those for cholesterol biosynthesis. We determined the crystal structures of S. aureus dehydrosqualene synthase (CrtM) at 1.58 angstrom resolution, finding structural similarity to human squalene synthase (SQS). We screened nine SQS inhibitors and determined the structures of three, bound to CrtM. One, previously tested for cholesterol-lowering activity in humans, blocked staphyloxanthin biosynthesis in vitro (median inhibitory concentration approximately 100 nM), resulting in colorless bacteria with increased susceptibility to killing by human blood and to innate immune clearance in a mouse infection model. This finding represents proof of principle for a virulence factor-based therapy against S. aureus.
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[[Category: Large Structures]]
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==About this Structure==
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2ZCO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCO OCA].
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==Reference==
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A cholesterol biosynthesis inhibitor blocks Staphylococcus aureus virulence., Liu CI, Liu GY, Song Y, Yin F, Hensler ME, Jeng WY, Nizet V, Wang AH, Oldfield E, Science. 2008 Mar 7;319(5868):1391-4. Epub 2008 Feb 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18276850 18276850]
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[[Category: Single protein]]
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[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Jeng, W Y.]]
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[[Category: Jeng WY]]
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[[Category: Liu, C I.]]
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[[Category: Liu CI]]
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[[Category: Oldfield, E.]]
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[[Category: Oldfield E]]
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[[Category: Wang, A H.]]
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[[Category: Wang AH]]
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[[Category: carotenoid biosynthesis]]
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[[Category: crtm]]
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[[Category: head-to-head condensation]]
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[[Category: staphyloxanthin biosynthesis]]
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[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:20:26 2008''
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Current revision

Crystal structure of the C(30) carotenoid dehydrosqualene synthase from Staphylococcus aureus

PDB ID 2zco

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