2zfd

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[[Image:2zfd.jpg|left|200px]]
 
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{{Structure
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==The crystal structure of plant specific calcium binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14==
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|PDB= 2zfd |SIZE=350|CAPTION= <scene name='initialview01'>2zfd</scene>, resolution 1.20&Aring;
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<StructureSection load='2zfd' size='340' side='right'caption='[[2zfd]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
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<table><tr><td colspan='2'>[[2zfd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZFD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZFD FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
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|GENE= CBL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]), CIPK14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zfd OCA], [https://pdbe.org/2zfd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zfd RCSB], [https://www.ebi.ac.uk/pdbsum/2zfd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zfd ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1uhn|1UHN]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zfd OCA], [http://www.ebi.ac.uk/pdbsum/2zfd PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2zfd RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/CNBL2_ARATH CNBL2_ARATH] Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Binds four calcium ions per subunit. Mediates the activation of AKT1 by CIPK proteins (CIPK6, CIPK16, and CIPK23) in response to low potassium conditions and in the context of stomatal movement. Mediates the inactivation of the proton pump AHA2 by CIPK11. Probably involved in regulating signaling responses to abscisic acid.<ref>PMID:17483306</ref> <ref>PMID:17898163</ref> <ref>PMID:22547024</ref>
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== Evolutionary Conservation ==
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'''The crystal structure of plant specific calcium binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zf/2zfd_consurf.spt"</scriptWhenChecked>
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Calcium signals mediate a multitude of plant responses to external stimuli. Calcineurin B-like (CBL) proteins and their target kinases, CBL-interacting protein kinases (CIPKs), represent important relays in plant calcium signaling. CBL interacts with CIPK through a conserved motif (NAF/FISL motif) within the C-terminal regulatory domain. To better understand the functional role of the CBL-CIPK system, we determined the crystal structure of AtCBL2 in complex with the regulatory domain of AtCIPK14 at 1.2 A resolution. The NAF/FISL motif is inserted into a hydrophobic crevice within AtCBL2, accompanied by a large displacement of the helices and loop on the opposite side of the NAF/FISL motif from the C-terminal region, which shields the hydrophobic crevice in free form. Ca(2+) are coordinated within four EF hands in AtCBL2 in bound form. This calcium coordination pattern differs from that in the structure of the SOS3-SOS2 complex previously reported. Structural comparison of the two structures shows that the recognition of CBL by CIPK is performed in a similar manner, but inherent interactions confer binding affinity and specificity.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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2ZFD is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZFD OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zfd ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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The Crystal Structure of Plant-Specific Calcium-Binding Protein AtCBL2 in Complex with the Regulatory Domain of AtCIPK14., Akaboshi M, Hashimoto H, Ishida H, Saijo S, Koizumi N, Sato M, Shimizu T, J Mol Biol. 2008 Jan 11;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18237745 18237745]
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<references/>
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__TOC__
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</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Akaboshi, M.]]
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[[Category: Akaboshi M]]
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[[Category: Hashimoto, H.]]
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[[Category: Hashimoto H]]
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[[Category: Ishida, H.]]
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[[Category: Ishida H]]
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[[Category: Koizumi, N.]]
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[[Category: Koizumi N]]
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[[Category: Sato, M.]]
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[[Category: Sato M]]
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[[Category: Shimizu, T.]]
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[[Category: Shimizu T]]
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[[Category: atp-binding]]
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[[Category: calcium binding protein]]
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[[Category: kinase]]
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[[Category: nucleotide-binding]]
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[[Category: protein-protein complex]]
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[[Category: serine/threonine-protein kinase]]
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[[Category: signaling protein/transferase complex]]
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[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:20:45 2008''
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Current revision

The crystal structure of plant specific calcium binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14

PDB ID 2zfd

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