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| | ==Crystal Structure of the Strawberry Pathogenesis-Related 10 (PR-10) Fra a 2 protein (A141F) processed with the CrystalDirect automated mounting and cryo-cooling technology== | | ==Crystal Structure of the Strawberry Pathogenesis-Related 10 (PR-10) Fra a 2 protein (A141F) processed with the CrystalDirect automated mounting and cryo-cooling technology== |
| - | <StructureSection load='5amw' size='340' side='right' caption='[[5amw]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='5amw' size='340' side='right'caption='[[5amw]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5amw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fraan Fraan]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AMW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AMW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5amw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Fragaria_x_ananassa Fragaria x ananassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AMW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AMW FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5amx|5amx]], [[5amy|5amy]], [[5amz|5amz]], [[5an2|5an2]], [[5an4|5an4]], [[5and|5and]], [[5ane|5ane]], [[5ang|5ang]], [[5ani|5ani]], [[5anj|5anj]], [[5ank|5ank]], [[5anl|5anl]], [[5ano|5ano]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Fraa2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3747 FRAAN])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5amw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5amw OCA], [https://pdbe.org/5amw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5amw RCSB], [https://www.ebi.ac.uk/pdbsum/5amw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5amw ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5amw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5amw OCA], [http://pdbe.org/5amw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5amw RCSB], [http://www.ebi.ac.uk/pdbsum/5amw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5amw ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/FRA12_FRAAN FRA12_FRAAN] Involved in the control of flavonoid biosynthesis in fruits, probably by binding directly to natural flavonoids (By similarity). Binds the natural flavonoid myricetin with affinities in the low micromolar range (PubMed:24133217).[UniProtKB:D0E0C7]<ref>PMID:24133217</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Fraan]] | + | [[Category: Fragaria x ananassa]] |
| - | [[Category: Casanal, A]] | + | [[Category: Large Structures]] |
| - | [[Category: Cipriani, F]] | + | [[Category: Casanal A]] |
| - | [[Category: Cornaciu, I]] | + | [[Category: Cipriani F]] |
| - | [[Category: Hoffmann, G]] | + | [[Category: Cornaciu I]] |
| - | [[Category: Marquez, J A]] | + | [[Category: Hoffmann G]] |
| - | [[Category: Pott, D]] | + | [[Category: Marquez JA]] |
| - | [[Category: Valpuesta, V]] | + | [[Category: Pott D]] |
| - | [[Category: Zander, U]] | + | [[Category: Valpuesta V]] |
| - | [[Category: Allergen]]
| + | [[Category: Zander U]] |
| - | [[Category: Automated cryo-cooling]]
| + | |
| - | [[Category: Automated crystal harvesting]]
| + | |
| - | [[Category: Bet v 1]]
| + | |
| - | [[Category: Crystaldirect]]
| + | |
| - | [[Category: Flavonoid]]
| + | |
| - | [[Category: Pyr/pyl/rcar]]
| + | |
| Structural highlights
Function
FRA12_FRAAN Involved in the control of flavonoid biosynthesis in fruits, probably by binding directly to natural flavonoids (By similarity). Binds the natural flavonoid myricetin with affinities in the low micromolar range (PubMed:24133217).[UniProtKB:D0E0C7][1]
Publication Abstract from PubMed
Currently, macromolecular crystallography projects often require the use of highly automated facilities for crystallization and X-ray data collection. However, crystal harvesting and processing largely depend on manual operations. Here, a series of new methods are presented based on the use of a low X-ray-background film as a crystallization support and a photoablation laser that enable the automation of major operations required for the preparation of crystals for X-ray diffraction experiments. In this approach, the controlled removal of the mother liquor before crystal mounting simplifies the cryocooling process, in many cases eliminating the use of cryoprotectant agents, while crystal-soaking experiments are performed through diffusion, precluding the need for repeated sample-recovery and transfer operations. Moreover, the high-precision laser enables new mounting strategies that are not accessible through other methods. This approach bridges an important gap in automation and can contribute to expanding the capabilities of modern macromolecular crystallography facilities.
Automated harvesting and processing of protein crystals through laser photoablation.,Zander U, Hoffmann G, Cornaciu I, Marquette JP, Papp G, Landret C, Seroul G, Sinoir J, Rower M, Felisaz F, Rodriguez-Puente S, Mariaule V, Murphy P, Mathieu M, Cipriani F, Marquez JA Acta Crystallogr D Struct Biol. 2016 Apr 1;72(Pt 4):454-66. doi:, 10.1107/S2059798316000954. Epub 2016 Mar 24. PMID:27050125[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Casanal A, Zander U, Munoz C, Dupeux F, Luque I, Botella MA, Schwab W, Valpuesta V, Marquez JA. The Strawberry Pathogenesis-Related 10 (PR-10) Fra a proteins control flavonoid biosynthesis by binding to metabolic intermediates. J Biol Chem. 2013 Oct 16. PMID:24133217 doi:http://dx.doi.org/10.1074/jbc.M113.501528
- ↑ Zander U, Hoffmann G, Cornaciu I, Marquette JP, Papp G, Landret C, Seroul G, Sinoir J, Rower M, Felisaz F, Rodriguez-Puente S, Mariaule V, Murphy P, Mathieu M, Cipriani F, Marquez JA. Automated harvesting and processing of protein crystals through laser photoablation. Acta Crystallogr D Struct Biol. 2016 Apr 1;72(Pt 4):454-66. doi:, 10.1107/S2059798316000954. Epub 2016 Mar 24. PMID:27050125 doi:http://dx.doi.org/10.1107/S2059798316000954
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