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5dlb

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==Crystal structure of chaperone EspG3 of ESX-3 type VII secretion system from Mycobacterium marinum M==
==Crystal structure of chaperone EspG3 of ESX-3 type VII secretion system from Mycobacterium marinum M==
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<StructureSection load='5dlb' size='340' side='right' caption='[[5dlb]], [[Resolution|resolution]] 1.77&Aring;' scene=''>
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<StructureSection load='5dlb' size='340' side='right'caption='[[5dlb]], [[Resolution|resolution]] 1.77&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5dlb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycmm Mycmm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DLB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DLB FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5dlb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_marinum_M Mycobacterium marinum M]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DLB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DLB FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.77&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MMAR_0548 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216594 MYCMM])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dlb OCA], [http://pdbe.org/5dlb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dlb RCSB], [http://www.ebi.ac.uk/pdbsum/5dlb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dlb ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dlb OCA], [https://pdbe.org/5dlb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dlb RCSB], [https://www.ebi.ac.uk/pdbsum/5dlb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dlb ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/B2HNX0_MYCMM B2HNX0_MYCMM]
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Type VII secretion systems (ESX) are responsible for transport of multiple proteins in mycobacteria. How different ESX systems achieve specific secretion of cognate substrates remains elusive. In the ESX systems, the cytoplasmic chaperone EspG forms complexes with heterodimeric PE-PPE substrates that are secreted from the cells or remain associated with the cell surface. Here we report the crystal structure of the EspG1 chaperone from the ESX-1 system determined using a fusion strategy with T4 lysozyme. EspG1 adopts a quasi 2-fold symmetric structure that consists of a central beta-sheet and two alpha-helical bundles. Additionally, we describe the structures of EspG3 chaperones from four different crystal forms. Alternate conformations of the putative PE-PPE binding site are revealed by comparison of the available EspG3 structures. Analysis of EspG1, EspG3 and EspG5 chaperones using small-angle X-ray scattering (SAXS) reveals that EspG1 and EspG3 chaperones form dimers in solution, which we observed in several of our crystal forms. Finally, we propose a model of the ESX-3 specific EspG3-PE5-PPE4 complex based on the SAXS analysis.
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Structural Variability of EspG Chaperones from Mycobacterial ESX-1, ESX-3 and ESX-5 Type VII Secretion Systems.,Tuukkanen AT, Freire D, Chan S, Arbing MA, Reed RW, Evans TJ, Zenkeviciute G, Kim J, Kahng S, Sawaya MR, Chaton CT, Wilmanns M, Eisenberg D, Parret AHA, Korotkov KV J Mol Biol. 2018 Nov 9. pii: S0022-2836(18)30423-6. doi:, 10.1016/j.jmb.2018.11.003. PMID:30419243<ref>PMID:30419243</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5dlb" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Mycmm]]
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[[Category: Large Structures]]
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[[Category: Arbing, M A]]
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[[Category: Mycobacterium marinum M]]
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[[Category: Chan, S]]
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[[Category: Arbing MA]]
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[[Category: Eisenberg, D S]]
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[[Category: Chan S]]
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[[Category: Kahng, S]]
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[[Category: Eisenberg DS]]
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[[Category: Kim, J]]
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[[Category: Kahng S]]
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[[Category: Sawaya, M R]]
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[[Category: Kim J]]
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[[Category: Structural genomic]]
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[[Category: Sawaya MR]]
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[[Category: Chaperone]]
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[[Category: Esx]]
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[[Category: PSI, Protein structure initiative]]
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[[Category: Secretion]]
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[[Category: Tbsgc]]
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[[Category: Virulence]]
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Current revision

Crystal structure of chaperone EspG3 of ESX-3 type VII secretion system from Mycobacterium marinum M

PDB ID 5dlb

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