|
|
| (One intermediate revision not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal structure of inner membrane protein Bqt4 in complex with LEM2== | | ==Crystal structure of inner membrane protein Bqt4 in complex with LEM2== |
| - | <StructureSection load='5yca' size='340' side='right' caption='[[5yca]], [[Resolution|resolution]] 1.57Å' scene=''> | + | <StructureSection load='5yca' size='340' side='right'caption='[[5yca]], [[Resolution|resolution]] 1.57Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5yca]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YCA FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5yca]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C] and [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YCA FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bqt4, SPBC19C7.10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), lem2, heh1, SPAC18G6.10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.57Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yca OCA], [http://pdbe.org/5yca PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yca RCSB], [http://www.ebi.ac.uk/pdbsum/5yca PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yca ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yca OCA], [https://pdbe.org/5yca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yca RCSB], [https://www.ebi.ac.uk/pdbsum/5yca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yca ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SMT3_YEAST SMT3_YEAST]] Not known; suppressor of MIF2 mutations. [[http://www.uniprot.org/uniprot/LEM2_SCHPO LEM2_SCHPO]] Nucleus inner membrane protein involved in meiosis.<ref>PMID:20404563</ref> | + | [https://www.uniprot.org/uniprot/BQT4_SCHPO BQT4_SCHPO] Connects telomeres to the nuclear envelop (NE) during both vegetative growth and meiosis. This connection ensures clustering of telomeres to the spindle pole body (SPB) when cells enter meiotic prophase.<ref>PMID:19948484</ref> [https://www.uniprot.org/uniprot/SMT3_YEAST SMT3_YEAST] Not known; suppressor of MIF2 mutations. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 22: |
Line 22: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Fission yeast]] | + | [[Category: Large Structures]] |
| - | [[Category: Chen, Y]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Hu, C]] | + | [[Category: Schizosaccharomyces pombe 972h-]] |
| - | [[Category: Bouquet formation]]
| + | [[Category: Chen Y]] |
| - | [[Category: Chromosome organization]]
| + | [[Category: Hu C]] |
| - | [[Category: Lap-emerin-man domain protein]]
| + | |
| - | [[Category: Membrane organization]] | + | |
| - | [[Category: Membrane protein]] | + | |
| Structural highlights
Function
BQT4_SCHPO Connects telomeres to the nuclear envelop (NE) during both vegetative growth and meiosis. This connection ensures clustering of telomeres to the spindle pole body (SPB) when cells enter meiotic prophase.[1] SMT3_YEAST Not known; suppressor of MIF2 mutations.
Publication Abstract from PubMed
The dynamic association of chromosomes with the nuclear envelope (NE) is essential for chromosome maintenance. Schizosaccharomyces pombe inner nuclear membrane protein Bqt4 plays a critical role in connecting telomeres to the NE, mainly through a direct interaction with the telomeric protein Rap1. Bqt4 also interacts with Lem2 for pericentric heterochromatin maintenance. How Bqt4 coordinates the interactions with different proteins to exert their functions is unclear. Here, we report the crystal structures of the N-terminal domain of Bqt4 in complexes with Bqt4-binding motifs from Rap1, Lem2, and Sad1. The structural, biochemical and cellular analyses reveal that the N-terminal domain of Bqt4 is a protein-interaction module that recognizes a consensus motif and plays essential roles in telomere-NE association and meiosis progression. Phosphorylation of Bqt4-interacting proteins may act as a switch to regulate these interactions during cell cycles. Our studies provide structural insights into the identification and regulation of Bqt4-mediated interactions.
Structural insights into chromosome attachment to the nuclear envelope by an inner nuclear membrane protein Bqt4 in fission yeast.,Hu C, Inoue H, Sun W, Takeshita Y, Huang Y, Xu Y, Kanoh J, Chen Y Nucleic Acids Res. 2018 Nov 20. pii: 5193554. doi: 10.1093/nar/gky1186. PMID:30462301[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chikashige Y, Yamane M, Okamasa K, Tsutsumi C, Kojidani T, Sato M, Haraguchi T, Hiraoka Y. Membrane proteins Bqt3 and -4 anchor telomeres to the nuclear envelope to ensure chromosomal bouquet formation. J Cell Biol. 2009 Nov 2;187(3):413-27. doi: 10.1083/jcb.200902122. PMID:19948484 doi:http://dx.doi.org/10.1083/jcb.200902122
- ↑ Hu C, Inoue H, Sun W, Takeshita Y, Huang Y, Xu Y, Kanoh J, Chen Y. Structural insights into chromosome attachment to the nuclear envelope by an inner nuclear membrane protein Bqt4 in fission yeast. Nucleic Acids Res. 2018 Nov 20. pii: 5193554. doi: 10.1093/nar/gky1186. PMID:30462301 doi:http://dx.doi.org/10.1093/nar/gky1186
|
