3f8r

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of Sulfolobus solfataricus Thioredoxin reductase B3 in complex with two NADP molecules

Structural highlights

3f8r is a 4 chain structure with sequence from Saccharolobus solfataricus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.95Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8X236_SACSO

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Recent investigations have demonstrated that disulfide bridges may play a crucial role in the stabilization of proteins in hyperthermophilic organisms. A major role in the process of disulfide formation is played by ubiquitous proteins belonging to the thioredoxin superfamily, which includes thioredoxins (Trx), thioredoxin reductases (TrxR), and disulfide oxidases/isomerases (PDO/PDI). Here we report a characterization of the structure and stability of the TrxR (SsTrxRB3) isolated from the archaeon Sulfolobus solfataricus. This protein is particularly interesting since it is able to process different substrates (Trxs and PDO) and it is endowed with an additional NADH oxidase activity. The crystal structure of the wild-type enzyme, of its complex with NADP and of the C147A mutant provides interesting clues on the enzyme function. In contrast to what is observed for class II TrxRs, in the structure of the oxidized enzyme, the FAD binding site is occupied by a partially disordered NAD molecule. In the active site of the C147A mutant, which exhibits a marginal NADH oxidase activity, the FAD is canonically bound to the enzyme. Molecular modeling indicates that a FAD molecule can be accommodated in the site of the reduced SsTrxRB3. Depending on the oxidation state, SsTrxRB3 can bind a different cofactor in its active site. This peculiar feature has been related to its dual activity. Denaturation experiments followed by circular dichroism indicate that electrostatic interactions play an important role in the stabilization of this thermostable protein. The analysis of the enzyme 3D-structure has also provided insights into the bases of SsTrxRB3 stability.

Structure and stability of a thioredoxin reductase from Sulfolobus solfataricus: a thermostable protein with two functions.,Ruggiero A, Masullo M, Ruocco MR, Grimaldi P, Lanzotti MA, Arcari P, Zagari A, Vitagliano L Biochim Biophys Acta. 2009 Mar;1794(3):554-62. Epub 2008 Dec 6. PMID:19110078^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ruggiero A, Masullo M, Ruocco MR, Grimaldi P, Lanzotti MA, Arcari P, Zagari A, Vitagliano L. Structure and stability of a thioredoxin reductase from Sulfolobus solfataricus: a thermostable protein with two functions. Biochim Biophys Acta. 2009 Mar;1794(3):554-62. Epub 2008 Dec 6. PMID:19110078 doi:10.1016/j.bbapap.2008.11.011

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3f8r"

@@ Line 1: / Line 1: @@
 ==Crystal structure of Sulfolobus solfataricus Thioredoxin reductase B3 in complex with two NADP molecules==
-<StructureSection load='3f8r' size='340' side='right' caption='[[3f8r]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
+<StructureSection load='3f8r' size='340' side='right'caption='[[3f8r]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3f8r]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F8R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3F8R FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3f8r]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F8R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3F8R FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3f8p|3f8p]], [[3f8d|3f8d]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thioredoxin-disulfide_reductase Thioredoxin-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3f8r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f8r OCA], [https://pdbe.org/3f8r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3f8r RCSB], [https://www.ebi.ac.uk/pdbsum/3f8r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3f8r ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3f8r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f8r OCA], [http://pdbe.org/3f8r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3f8r RCSB], [http://www.ebi.ac.uk/pdbsum/3f8r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3f8r ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8X236_SACSO Q8X236_SACSO]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 14: / Line 15: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f8/3f8r_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 30: / Line 31: @@
 ==See Also==
-*[[Thioredoxin Reductase|Thioredoxin Reductase]]
+*[[Thioredoxin reductase 3D structures|Thioredoxin reductase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Atcc 35091]]
+[[Category: Large Structures]]
-[[Category: Thioredoxin-disulfide reductase]]
+[[Category: Saccharolobus solfataricus]]
-[[Category: Arcari, P]]
+[[Category: Arcari P]]
-[[Category: Masullo, M]]
+[[Category: Masullo M]]
-[[Category: Ruggiero, A]]
+[[Category: Ruggiero A]]
-[[Category: Ruocco, M R]]
+[[Category: Ruocco MR]]
-[[Category: Vitagliano, L]]
+[[Category: Vitagliano L]]
-[[Category: Zagari, A]]
+[[Category: Zagari A]]
-[[Category: Fad]]
-[[Category: Flavoprotein]]
-[[Category: Nucleotide binding]]
-[[Category: Oxidoreductase]]
-[[Category: Redox protein]]

3f8r

From Proteopedia

Current revision

Crystal structure of Sulfolobus solfataricus Thioredoxin reductase B3 in complex with two NADP molecules

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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