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| | ==Structural insight into the quinolone-DNA cleavage complex of type IIA topoisomerases== | | ==Structural insight into the quinolone-DNA cleavage complex of type IIA topoisomerases== |
| - | <StructureSection load='3fof' size='340' side='right' caption='[[3fof]], [[Resolution|resolution]] 4.00Å' scene=''> | + | <StructureSection load='3fof' size='340' side='right'caption='[[3fof]], [[Resolution|resolution]] 4.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3fof]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FOF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3fof]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FOF FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MFX:1-CYCLOPROPYL-6-FLUORO-8-METHOXY-7-[(4AS,7AS)-OCTAHYDRO-6H-PYRROLO[3,4-B]PYRIDIN-6-YL]-4-OXO-1,4-DIHYDROQUINOLINE-3-CARBOXYLIC+ACID'>MFX</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2nov|2nov]], [[3foe|3foe]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MFX:1-CYCLOPROPYL-6-FLUORO-8-METHOXY-7-[(4AS,7AS)-OCTAHYDRO-6H-PYRROLO[3,4-B]PYRIDIN-6-YL]-4-OXO-1,4-DIHYDROQUINOLINE-3-CARBOXYLIC+ACID'>MFX</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">parC, SP_0855 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 "Diplococcus pneumoniae" (Klein 1884) Weichselbaum 1886]), parE, SP_0852 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 "Diplococcus pneumoniae" (Klein 1884) Weichselbaum 1886])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fof OCA], [https://pdbe.org/3fof PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fof RCSB], [https://www.ebi.ac.uk/pdbsum/3fof PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fof ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fof OCA], [http://pdbe.org/3fof PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fof RCSB], [http://www.ebi.ac.uk/pdbsum/3fof PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fof ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PARC_STRPN PARC_STRPN]] Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.<ref>PMID:17375187</ref> <ref>PMID:20596531</ref> [[http://www.uniprot.org/uniprot/PARE_STRPN PARE_STRPN]] Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.<ref>PMID:17375187</ref> <ref>PMID:20596531</ref> | + | [https://www.uniprot.org/uniprot/PARC_STRPN PARC_STRPN] Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.<ref>PMID:17375187</ref> <ref>PMID:20596531</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Topoisomerase|Topoisomerase]] | + | *[[Topoisomerase 3D structures|Topoisomerase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Fisher, L M]] | + | [[Category: Large Structures]] |
| - | [[Category: Laponogov, I]]
| + | |
| - | [[Category: McAuley, K E]]
| + | |
| - | [[Category: Pan, X S]]
| + | |
| - | [[Category: Sanderson, M R]]
| + | |
| - | [[Category: Sawhney, R]]
| + | |
| - | [[Category: Sohi, M K]]
| + | |
| - | [[Category: Thompson, A W]]
| + | |
| - | [[Category: Veselkov, D A]]
| + | |
| - | [[Category: Atp-binding]]
| + | |
| - | [[Category: Cell membrane]]
| + | |
| - | [[Category: Dna]]
| + | |
| - | [[Category: Dna-binding]]
| + | |
| - | [[Category: Isomerase]]
| + | |
| - | [[Category: Isomerase-dna complex]]
| + | |
| - | [[Category: Membrane]]
| + | |
| - | [[Category: Moxifloxacin]]
| + | |
| - | [[Category: Nucleotide-binding]]
| + | |
| - | [[Category: Protein-dna cleavage complex]]
| + | |
| - | [[Category: Quinolone]]
| + | |
| | [[Category: Streptococcus pneumoniae]] | | [[Category: Streptococcus pneumoniae]] |
| - | [[Category: Topoisomerase]] | + | [[Category: Fisher LM]] |
| | + | [[Category: Laponogov I]] |
| | + | [[Category: McAuley KE]] |
| | + | [[Category: Pan X-S]] |
| | + | [[Category: Sanderson MR]] |
| | + | [[Category: Sawhney R]] |
| | + | [[Category: Sohi MK]] |
| | + | [[Category: Thompson AW]] |
| | + | [[Category: Veselkov DA]] |
| Structural highlights
Function
PARC_STRPN Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Type II topoisomerases alter DNA topology by forming a covalent DNA-cleavage complex that allows DNA transport through a double-stranded DNA break. We present the structures of cleavage complexes formed by the Streptococcus pneumoniae ParC breakage-reunion and ParE TOPRIM domains of topoisomerase IV stabilized by moxifloxacin and clinafloxacin, two antipneumococcal fluoroquinolones. These structures reveal two drug molecules intercalated at the highly bent DNA gate and help explain antibacterial quinolone action and resistance.
Structural insight into the quinolone-DNA cleavage complex of type IIA topoisomerases.,Laponogov I, Sohi MK, Veselkov DA, Pan XS, Sawhney R, Thompson AW, McAuley KE, Fisher LM, Sanderson MR Nat Struct Mol Biol. 2009 Jun;16(6):667-9. Epub 2009 May 17. PMID:19448616[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Laponogov I, Veselkov DA, Sohi MK, Pan XS, Achari A, Yang C, Ferrara JD, Fisher LM, Sanderson MR. Breakage-reunion domain of Streptococcus pneumoniae topoisomerase IV: crystal structure of a gram-positive quinolone target. PLoS ONE. 2007 Mar 21;2(3):e301. PMID:17375187 doi:10.1371/journal.pone.0000301
- ↑ Laponogov I, Pan XS, Veselkov DA, McAuley KE, Fisher LM, Sanderson MR. Structural basis of gate-DNA breakage and resealing by type II topoisomerases. PLoS One. 2010 Jun 28;5(6):e11338. PMID:20596531 doi:10.1371/journal.pone.0011338
- ↑ Laponogov I, Sohi MK, Veselkov DA, Pan XS, Sawhney R, Thompson AW, McAuley KE, Fisher LM, Sanderson MR. Structural insight into the quinolone-DNA cleavage complex of type IIA topoisomerases. Nat Struct Mol Biol. 2009 Jun;16(6):667-9. Epub 2009 May 17. PMID:19448616 doi:10.1038/nsmb.1604
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