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| - | [[Image:332d.gif|left|200px]] | |
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| - | {{Structure
| + | ==CRYSTAL STRUCTURE OF R(GUGUGUA)D(C) WITH TANDEM G-U/U-G WOBBLE PAIRS WITH STRAND SLIPPAGE== |
| - | |PDB= 332d |SIZE=350|CAPTION= <scene name='initialview01'>332d</scene>, resolution 1.580Å
| + | <StructureSection load='332d' size='340' side='right'caption='[[332d]], [[Resolution|resolution]] 1.58Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>
| + | <table><tr><td colspan='2'>[[332d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=332D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=332D FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.58Å</td></tr> |
| - | |GENE=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=332d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=332d OCA], [https://pdbe.org/332d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=332d RCSB], [https://www.ebi.ac.uk/pdbsum/332d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=332d ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | __TOC__ |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=332d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=332d OCA], [http://www.ebi.ac.uk/pdbsum/332d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=332d RCSB]</span>
| + | </StructureSection> |
| - | }}
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Biswas R]] |
| - | '''CRYSTAL STRUCTURE OF R(GUGUGUA)D(C) WITH TANDEM G-U/U-G WOBBLE PAIRS WITH STRAND SLIPPAGE'''
| + | [[Category: Sundaralingam M]] |
| - | | + | |
| - | | + | |
| - | ==Overview==
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| - | To better understand the frequent occurrence of adjacent wobble pairs in ribosomal RNAs we have determined the crystal structure of the RNA duplex, r(GUGUGUA)dC with the 3'-terminal deoxy C residue. Two different crystal forms of the duplex were obtained and both belong to the rhombohedral space group, R3. Crystal form I has hexagonal unit cell dimensions, a = b = 40.82 A and c = 66.09 A and diffracts to 1.58 A resolution, while crystal form II has a = b = 47.11 A and c = 59.86 A, diffracting only to 2.50 A resolution. Both structures were solved by the molecular replacement method using different starting models. In spite of the large differences in the cell dimensions the overall structures in both crystals are similar. Instead of the expected blunt-end duplex with four consecutive G x U pairs, the slippage of the strands resulted in two different tandem G x U/U x G wobble pairs involving two of the central and two of the 5' overhang bases, still yielding a total of four wobble pairs. These tandem wobble pairs are flanked by two Watson-Crick pairs. The A-type duplexes stack in the familiar head-to-tail fashion forming a pseudocontinuous helix. The wobble pairs of the present motif II (G x U/U x G) structure stack with a low twist angle of 25.3 degrees in contrast to that of motif I (U x G/G x U), 38.1 degrees. The four wobble pairs are characteristically heavily hydrated in both the grooves accounting for their stability.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 332D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=332D OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Crystal structure of r(GUGUGUA)dC with tandem G x U/U x G wobble pairs with strand slippage., Biswas R, Sundaralingam M, J Mol Biol. 1997 Jul 18;270(3):511-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9237915 9237915]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Biswas, R.]] | + | |
| - | [[Category: Sundaralingam, M.]] | + | |
| - | [[Category: a-dna/rna]]
| + | |
| - | [[Category: double helix]]
| + | |
| - | [[Category: overhanging base]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:21:26 2008''
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