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| - | [[Image:335d.gif|left|200px]] | |
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| - | {{Structure
| + | ==THE CRYSTAL STRUCTURE OF THE DNA DECAMER D(GGCAATTGCG) CONTAINS BOTH MAJOR AND MINOR GROOVE BINDING G.(G.C) TRIPLETS== |
| - | |PDB= 335d |SIZE=350|CAPTION= <scene name='initialview01'>335d</scene>, resolution 2.400Å
| + | <StructureSection load='335d' size='340' side='right'caption='[[335d]], [[Resolution|resolution]] 2.40Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
| + | <table><tr><td colspan='2'>[[335d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=335D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=335D FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=335d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=335d OCA], [https://pdbe.org/335d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=335d RCSB], [https://www.ebi.ac.uk/pdbsum/335d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=335d ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=335d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=335d OCA], [http://www.ebi.ac.uk/pdbsum/335d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=335d RCSB]</span>
| + | __TOC__ |
| - | }}
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | '''THE CRYSTAL STRUCTURE OF THE DNA DECAMER D(GGCAATTGCG) CONTAINS BOTH MAJOR AND MINOR GROOVE BINDING G.(G.C) TRIPLETS'''
| + | [[Category: Neidle S]] |
| - | | + | [[Category: Nunn CM]] |
| - | | + | |
| - | ==Overview==
| + | |
| - | The crystal structure of the decamer d(GGCAATTGCG) has been determined at 2.4 A resolution. The central eight bases of each DNA single-strand base pair with a self-complementary strand to form an octamer B-DNA duplex. These duplexes lie end-to-end within the unit cell. The terminal 5'-G and G-3' bases of each decamer strand are unpaired, and interact with the neighbouring duplexes via interactions within both the major and minor groove. This results in base triplets of the type G-(G.C) and G*(G.C), with the third guanine base binding to a Watson-Crick G.C base pair from the major groove and the minor groove, respectively. The triplet interaction of the type G-(G.C) involves Hoogsteen hydrogen-bonding interactions between the two guanine bases. The minor- and major-groove base triplet interactions which exist within this structure act to stabilize the d(GCAATTGC)2 B-DNA octamer duplex.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 335D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=335D OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Structure of the DNA decamer d(GGCAATTGCG) contains both major- and minor-groove binding G.(G.C) base triplets., Nunn CM, Neidle S, Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):577-83. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9761852 9761852]
| + | |
| - | [[Category: Protein complex]] | + | |
| - | [[Category: Neidle, S.]] | + | |
| - | [[Category: Nunn, C M.]] | + | |
| - | [[Category: a-dna]]
| + | |
| - | [[Category: double helix]]
| + | |
| - | [[Category: flipped-out base]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:21:31 2008''
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