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| - | [[Image:3ana.jpg|left|200px]] | |
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| - | {{Structure
| + | ==THREE-DIMENSIONAL STRUCTURE OF D(GGGATCCC) IN THE CRYSTALLINE STATE== |
| - | |PDB= 3ana |SIZE=350|CAPTION= <scene name='initialview01'>3ana</scene>, resolution 2.500Å
| + | <StructureSection load='3ana' size='340' side='right'caption='[[3ana]], [[Resolution|resolution]] 2.50Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>
| + | <table><tr><td colspan='2'>[[3ana]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ANA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ANA FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | |GENE=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ana FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ana OCA], [https://pdbe.org/3ana PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ana RCSB], [https://www.ebi.ac.uk/pdbsum/3ana PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ana ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | __TOC__ |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ana FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ana OCA], [http://www.ebi.ac.uk/pdbsum/3ana PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3ana RCSB]</span>
| + | </StructureSection> |
| - | }}
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Heinemann U]] |
| - | '''THREE-DIMENSIONAL STRUCTURE OF D(GGGATCCC) IN THE CRYSTALLINE STATE'''
| + | [[Category: Lauble H]] |
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| - | | + | |
| - | ==Overview==
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| - | The structure of the self-complementary octamer d(GGGATCCC) has been analysed by single crystal X-ray diffraction methods at a nominal resolution of 2.5 A. With acceptable stereochemistry of the model the crystallographic R factor was 16.6% after restrained least-squares refinement. In the crystal, d(GGGATCCC) forms an A-DNA double helix with slightly varying conformation of the two strands. The average displacement of the base pairs from the helix axis is unusually large and is accompanied by pronounced sliding of the base pairs along their long axes at all dinucleotide steps except for the central AT. With 12 base pairs per complete turn the helix is considerably underwound. As observed with most oligodeoxyribonucleotides analysed by X-ray crystallography so far, the octamer displays reduced base pair tilt, increased rise per base pair and a more open major groove compared with canonical A-DNA. We propose that, based on these parameters, three A-helical sub-families may be defined; d(GGGATCCC) then is a representative of the class with intermediate tilt, rise, and major groove width.
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| - | ==About this Structure==
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| - | 3ANA is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ANA OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Three-dimensional structure of d(GGGATCCC) in the crystalline state., Lauble H, Frank R, Blocker H, Heinemann U, Nucleic Acids Res. 1988 Aug 25;16(16):7799-816. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3166518 3166518]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Heinemann, U.]] | + | |
| - | [[Category: Lauble, H.]] | + | |
| - | [[Category: a-dna]]
| + | |
| - | [[Category: double helix]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:22:25 2008''
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