3gb7

From Proteopedia

(Difference between revisions)

Current revision

Potassium Channel KcsA-Fab complex in Li+

Structural highlights

3gb7 is a 3 chain structure with sequence from Mus musculus and Streptomyces lividans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.85Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KCSA_STRLI Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel, although it is not clear if this is the physiological stimulus for channel opening. Monovalent cation preference is K(+) > Rb(+) > NH4(+) >> Na(+) > Li(+).^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Potassium channels allow K(+) ions to diffuse through their pores while preventing smaller Na(+) ions from permeating. Discrimination between these similar, abundant ions enables these proteins to control electrical and chemical activity in all organisms. Selection occurs at the narrow selectivity filter containing structurally identified K(+) binding sites. Selectivity is thought to arise because smaller ions such as Na(+) do not bind to these K(+) sites in a thermodynamically favorable way. Using the model K(+) channel KcsA, we examined how intracellular Na(+) and Li(+) interact with the pore and the permeant ions using electrophysiology, molecular dynamics simulations and X-ray crystallography. Our results suggest that these small cations have a separate binding site within the K(+) selectivity filter. We propose that selective permeation from the intracellular side primarily results from a large energy barrier blocking filter entry for Na(+) and Li(+) in the presence of K(+), not from a difference of binding affinity between ions.

Mechanism of potassium-channel selectivity revealed by Na(+) and Li(+) binding sites within the KcsA pore.,Thompson AN, Kim I, Panosian TD, Iverson TM, Allen TW, Nimigean CM Nat Struct Mol Biol. 2009 Dec;16(12):1317-24. Epub 2009 Nov 29. PMID:19946269^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Schrempf H, Schmidt O, Kummerlen R, Hinnah S, Muller D, Betzler M, Steinkamp T, Wagner R. A prokaryotic potassium ion channel with two predicted transmembrane segments from Streptomyces lividans. EMBO J. 1995 Nov 1;14(21):5170-8. PMID:7489706
↑ Thompson AN, Kim I, Panosian TD, Iverson TM, Allen TW, Nimigean CM. Mechanism of potassium-channel selectivity revealed by Na(+) and Li(+) binding sites within the KcsA pore. Nat Struct Mol Biol. 2009 Dec;16(12):1317-24. Epub 2009 Nov 29. PMID:19946269 doi:10.1038/nsmb.1703

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3gb7"

@@ Line 1: / Line 1: @@
 ==Potassium Channel KcsA-Fab complex in Li+==
-<StructureSection load='3gb7' size='340' side='right' caption='[[3gb7]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
+<StructureSection load='3gb7' size='340' side='right'caption='[[3gb7]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3gb7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_lividans"_krasil'nikov_et_al._1965 "actinomyces lividans" krasil'nikov et al. 1965] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GB7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3gb7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GB7 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">kcsA, skc1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1916 "Actinomyces lividans" Krasil'nikov et al. 1965])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gb7 OCA], [http://pdbe.org/3gb7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gb7 RCSB], [http://www.ebi.ac.uk/pdbsum/3gb7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3gb7 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gb7 OCA], [https://pdbe.org/3gb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gb7 RCSB], [https://www.ebi.ac.uk/pdbsum/3gb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gb7 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/KCSA_STRLI KCSA_STRLI]] Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel, although it is not clear if this is the physiological stimulus for channel opening. Monovalent cation preference is K(+) > Rb(+) > NH4(+) >> Na(+) > Li(+).<ref>PMID:7489706</ref>
+[https://www.uniprot.org/uniprot/KCSA_STRLI KCSA_STRLI] Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel, although it is not clear if this is the physiological stimulus for channel opening. Monovalent cation preference is K(+) > Rb(+) > NH4(+) >> Na(+) > Li(+).<ref>PMID:7489706</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 15: / Line 15: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gb/3gb7_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 32: / Line 32: @@
 ==See Also==
 *[[Antibody 3D structures|Antibody 3D structures]]
-*[[Potassium Channel|Potassium Channel]]
+*[[Potassium channel 3D structures|Potassium channel 3D structures]]
+*[[3D structures of non-human antibody|3D structures of non-human antibody]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Actinomyces lividans krasil'nikov et al. 1965]]
+[[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Allen, T W]]
+[[Category: Streptomyces lividans]]
-[[Category: Ilsoo, K]]
+[[Category: Allen TW]]
-[[Category: Iverson, T M]]
+[[Category: Ilsoo K]]
-[[Category: Nimigean, C M]]
+[[Category: Iverson TM]]
-[[Category: Panosian, T D]]
+[[Category: Nimigean CM]]
-[[Category: Thompson, A N]]
+[[Category: Panosian TD]]
-[[Category: Cell membrane]]
+[[Category: Thompson AN]]
-[[Category: Integral membrane protein]]
-[[Category: Ion selectivity]]
-[[Category: Ion transport]]
-[[Category: Ion-channel]]
-[[Category: Ionic channel]]
-[[Category: Lithium block]]
-[[Category: Membrane]]
-[[Category: Membrane protein]]
-[[Category: Potassium transport]]
-[[Category: Transmembrane]]
-[[Category: Transport]]
-[[Category: Voltage-gated channel]]
-[[Category: Voltage-gated potassium channel]]

3gb7

From Proteopedia

Current revision

Potassium Channel KcsA-Fab complex in Li+

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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