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3b4a

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[[Image:3b4a.gif|left|200px]]
 
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{{Structure
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==T. tengcongensis glmS ribozyme with G40A mutation, bound to glucosamine-6-phosphate==
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|PDB= 3b4a |SIZE=350|CAPTION= <scene name='initialview01'>3b4a</scene>, resolution 2.7&Aring;
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<StructureSection load='3b4a' size='340' side='right'caption='[[3b4a]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=A23:ADENOSINE-5&#39;-PHOSPHATE-2&#39;,3&#39;-CYCLIC+PHOSPHATE'>A23</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=GLP:GLUCOSAMINE+6-PHOSPHATE'>GLP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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<table><tr><td colspan='2'>[[3b4a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B4A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B4A FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A23:ADENOSINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>A23</scene>, <scene name='pdbligand=GLP:GLUCOSAMINE+6-PHOSPHATE'>GLP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b4a OCA], [https://pdbe.org/3b4a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b4a RCSB], [https://www.ebi.ac.uk/pdbsum/3b4a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b4a ProSAT]</span></td></tr>
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|RELATEDENTRY=[[2z74|2z74]], [[2z75|2z75]], [[2ho7|2ho7]], [[3b4b|3B4B]], [[3b4c|3B4C]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b4a OCA], [http://www.ebi.ac.uk/pdbsum/3b4a PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3b4a RCSB]</span>
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}}
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'''T. tengcongensis glmS ribozyme with G40A mutation, bound to glucosamine-6-phosphate'''
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==See Also==
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*[[Ribozyme 3D structures|Ribozyme 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The glmS ribozyme is a catalytic riboswitch that is activated for endonucleolytic cleavage by the coenzyme glucosamine-6-phosphate. Using kinetic assays and X-ray crystallography, we identify an active-site mutation of a conserved guanine that abolishes catalysis without perturbing coenzyme binding. Our results provide evidence that coenzyme function requires a specific nucleobase to interact with the nucleophile of the cleavage reaction.
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[[Category: Caldanaerobacter subterraneus subsp. tengcongensis]]
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[[Category: Large Structures]]
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==About this Structure==
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[[Category: Ferre-D'Amare AR]]
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3B4A is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermoanaerobacter_tengcongensis Thermoanaerobacter tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B4A OCA].
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[[Category: Klein DJ]]
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==Reference==
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Essential role of an active-site guanine in glmS ribozyme catalysis., Klein DJ, Been MD, Ferre-D'Amare AR, J Am Chem Soc. 2007 Dec 5;129(48):14858-9. Epub 2007 Nov 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17990888 17990888]
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[[Category: Protein complex]]
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[[Category: Thermoanaerobacter tengcongensis]]
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[[Category: Amare, A R.Ferre-D.]]
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[[Category: Klein, D J.]]
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[[Category: glucosamine-6-phosphate]]
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[[Category: riboswitch]]
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[[Category: ribozyme]]
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[[Category: rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:22:55 2008''
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Current revision

T. tengcongensis glmS ribozyme with G40A mutation, bound to glucosamine-6-phosphate

PDB ID 3b4a

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