3b74

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[[Image:3b74.jpg|left|200px]]
 
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{{Structure
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==Crystal Structure of Yeast Sec14 Homolog Sfh1 in Complex with Phosphatidylethanolamine==
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|PDB= 3b74 |SIZE=350|CAPTION= <scene name='initialview01'>3b74</scene>, resolution 1.90&Aring;
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<StructureSection load='3b74' size='340' side='right'caption='[[3b74]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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|SITE= <scene name='pdbsite=AC1:Pee+Binding+Site+For+Residue+A+311'>AC1</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=PEE:PHOSPHATIDYLETHANOLAMINE'>PEE</scene>
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<table><tr><td colspan='2'>[[3b74]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B74 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEE:1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE'>PEE</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b74 OCA], [https://pdbe.org/3b74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b74 RCSB], [https://www.ebi.ac.uk/pdbsum/3b74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b74 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[3b7n|3B7N]], [[3b7q|3B7Q]], [[3b7z|3B7Z]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b74 OCA], [http://www.ebi.ac.uk/pdbsum/3b74 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3b74 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/YKJ1_YEAST YKJ1_YEAST]
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== Evolutionary Conservation ==
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'''Crystal Structure of Yeast Sec14 Homolog Sfh1 in Complex with Phosphatidylethanolamine'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b7/3b74_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b74 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Sec14, the major yeast phosphatidylinositol (PtdIns)/phosphatidylcholine (PtdCho) transfer protein, regulates essential interfaces between lipid metabolism and membrane trafficking from the trans-Golgi network (TGN). How Sec14 does so remains unclear. We report that Sec14 binds PtdIns and PtdCho at distinct (but overlapping) sites, and both PtdIns- and PtdCho-binding activities are essential Sec14 activities. We further show both activities must reside within the same molecule to reconstitute a functional Sec14 and for effective Sec14-mediated regulation of phosphoinositide homeostasis in vivo. This regulation is uncoupled from PtdIns-transfer activity and argues for an interfacial presentation mode for Sec14-mediated potentiation of PtdIns kinases. Such a regulatory role for Sec14 is a primary counter to action of the Kes1 sterol-binding protein that antagonizes PtdIns 4-OH kinase activity in vivo. Collectively, these findings outline functional mechanisms for the Sec14 superfamily and reveal additional layers of complexity for regulating phosphoinositide homeostasis in eukaryotes.
Sec14, the major yeast phosphatidylinositol (PtdIns)/phosphatidylcholine (PtdCho) transfer protein, regulates essential interfaces between lipid metabolism and membrane trafficking from the trans-Golgi network (TGN). How Sec14 does so remains unclear. We report that Sec14 binds PtdIns and PtdCho at distinct (but overlapping) sites, and both PtdIns- and PtdCho-binding activities are essential Sec14 activities. We further show both activities must reside within the same molecule to reconstitute a functional Sec14 and for effective Sec14-mediated regulation of phosphoinositide homeostasis in vivo. This regulation is uncoupled from PtdIns-transfer activity and argues for an interfacial presentation mode for Sec14-mediated potentiation of PtdIns kinases. Such a regulatory role for Sec14 is a primary counter to action of the Kes1 sterol-binding protein that antagonizes PtdIns 4-OH kinase activity in vivo. Collectively, these findings outline functional mechanisms for the Sec14 superfamily and reveal additional layers of complexity for regulating phosphoinositide homeostasis in eukaryotes.
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==About this Structure==
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Functional anatomy of phospholipid binding and regulation of phosphoinositide homeostasis by proteins of the sec14 superfamily.,Schaaf G, Ortlund EA, Tyeryar KR, Mousley CJ, Ile KE, Garrett TA, Ren J, Woolls MJ, Raetz CR, Redinbo MR, Bankaitis VA Mol Cell. 2008 Feb 1;29(2):191-206. PMID:18243114<ref>PMID:18243114</ref>
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3B74 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B74 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Functional anatomy of phospholipid binding and regulation of phosphoinositide homeostasis by proteins of the sec14 superfamily., Schaaf G, Ortlund EA, Tyeryar KR, Mousley CJ, Ile KE, Garrett TA, Ren J, Woolls MJ, Raetz CR, Redinbo MR, Bankaitis VA, Mol Cell. 2008 Feb 1;29(2):191-206. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18243114 18243114]
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</div>
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<div class="pdbe-citations 3b74" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Bankaitis V]]
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[[Category: Bankaitis, V.]]
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[[Category: Ortlund EA]]
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[[Category: Ortlund, E A.]]
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[[Category: Redinbo MR]]
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[[Category: Redinbo, M R.]]
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[[Category: Schaaf G]]
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[[Category: Schaaf, G.]]
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[[Category: golgi]]
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[[Category: phosphatidylethanolamine]]
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[[Category: phospholipid]]
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[[Category: sec14]]
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[[Category: signaling protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:23:38 2008''
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Current revision

Crystal Structure of Yeast Sec14 Homolog Sfh1 in Complex with Phosphatidylethanolamine

PDB ID 3b74

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