6ibq
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6ibq is ON HOLD Authors: de Wijn, R., Olieric, V., Lorber, B., Sauter, C. Description: Structure of a nonameric RNA duplex at room temperature in C...) |
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- | '''Unreleased structure''' | ||
- | + | ==Structure of a nonameric RNA duplex at room temperature in ChipX microfluidic device== | |
+ | <StructureSection load='6ibq' size='340' side='right'caption='[[6ibq]], [[Resolution|resolution]] 1.55Å' scene=''> | ||
+ | == Structural highlights == | ||
+ | <table><tr><td colspan='2'>[[6ibq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus Rattus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IBQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IBQ FirstGlance]. <br> | ||
+ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55Å</td></tr> | ||
+ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
+ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ibq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ibq OCA], [https://pdbe.org/6ibq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ibq RCSB], [https://www.ebi.ac.uk/pdbsum/6ibq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ibq ProSAT]</span></td></tr> | ||
+ | </table> | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | Determining optimal conditions for the production of well diffracting crystals is a key step in every biocrystallography project. Here, a microfluidic device is described that enables the production of crystals by counter-diffusion and their direct on-chip analysis by serial crystallography at room temperature. Nine 'non-model' and diverse biomacromolecules, including seven soluble proteins, a membrane protein and an RNA duplex, were crystallized and treated on-chip with a variety of standard techniques including micro-seeding, crystal soaking with ligands and crystal detection by fluorescence. Furthermore, the crystal structures of four proteins and an RNA were determined based on serial data collected on four synchrotron beamlines, demonstrating the general applicability of this multipurpose chip concept. | ||
- | + | A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography.,de Wijn R, Hennig O, Roche J, Engilberge S, Rollet K, Fernandez-Millan P, Brillet K, Betat H, Morl M, Roussel A, Girard E, Mueller-Dieckmann C, Fox GC, Olieric V, Gavira JA, Lorber B, Sauter C IUCrJ. 2019 Apr 19;6(Pt 3):454-464. doi: 10.1107/S2052252519003622. eCollection, 2019 May 1. PMID:31098026<ref>PMID:31098026</ref> | |
- | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
- | [[Category: | + | </div> |
- | [[Category: | + | <div class="pdbe-citations 6ibq" style="background-color:#fffaf0;"></div> |
- | [[Category: | + | == References == |
- | [[Category: | + | <references/> |
- | [[Category: De Wijn | + | __TOC__ |
+ | </StructureSection> | ||
+ | [[Category: Large Structures]] | ||
+ | [[Category: Rattus]] | ||
+ | [[Category: Lorber B]] | ||
+ | [[Category: Olieric V]] | ||
+ | [[Category: Sauter C]] | ||
+ | [[Category: De Wijn R]] |
Current revision
Structure of a nonameric RNA duplex at room temperature in ChipX microfluidic device
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Categories: Large Structures | Rattus | Lorber B | Olieric V | Sauter C | De Wijn R