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5t7m

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==LIGAND BINDING DOMAIN OF PSEUDOMONAS AERUGINOSA PAO1 AMINO ACID CHEMORECEPTOR PCTA IN COMPLEX WITH L-TRP==
==LIGAND BINDING DOMAIN OF PSEUDOMONAS AERUGINOSA PAO1 AMINO ACID CHEMORECEPTOR PCTA IN COMPLEX WITH L-TRP==
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<StructureSection load='5t7m' size='340' side='right' caption='[[5t7m]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
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<StructureSection load='5t7m' size='340' side='right'caption='[[5t7m]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5t7m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T7M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T7M FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5t7m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T7M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T7M FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5t65|5t65]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pctA_1, mcpB_2, AO946_32780, AOY09_01348, PAERUG_P32_London_17_VIM_2_10_11_00198 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t7m OCA], [https://pdbe.org/5t7m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t7m RCSB], [https://www.ebi.ac.uk/pdbsum/5t7m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t7m ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t7m OCA], [http://pdbe.org/5t7m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t7m RCSB], [http://www.ebi.ac.uk/pdbsum/5t7m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t7m ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/PCTA_PSEAE PCTA_PSEAE] Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. Major receptor that responds to all naturally occurring L-amino acids, except L-Gln and L-Asp. Also involved in repellent responses to trichloroethylene (TCE), chloroform and methylthiocyanate.<ref>PMID:16233808</ref> <ref>PMID:23650915</ref> <ref>PMID:8522505</ref> <ref>PMID:9353923</ref>
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Pseudomonas aeruginosa is an opportunistic pathogen and one of the major model organisms for the study of chemotaxis. The bacterium harbours 26 genes encoding chemoreceptors, most of which have not been annotated with a function. The paralogous chemoreceptors PctA and PctB (Pseudomonas chemotactic transducer A and B) were found to mediate chemotaxis towards L-amino acids. However, the ligand spectrum of the receptors is quite different since the recombinant ligand-binding region (LBR) of PctA binds 17 different L-amino acids whereas that of PctB recognizes only five. To determine the molecular basis underlying this ligand specificity, PctA-LBR and PctB-LBR have been purified and crystals have been produced after pre-incubation with L-Ile and L-Arg, respectively. Initial crystallization conditions have been identified by the counter-diffusion method and X-ray data have been collected at 2.5 A (PctA-LBR bound to L-Ile) and 3.14 A (PctB-LBR bound to L-Arg) resolution. Crystals belonged to space groups P2(1)2(1)2(1) and P3(1)2(1), with unit-cell parameters a = 72.2, b = 78.5, c = 116.6 A and a = b = 111.6, c = 117.4, respectively, for PctA-LBR and PctB-LBR. Molecular-replacement methods will be pursued for structural determination.
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Purification, crystallization and preliminary crystallographic analysis of the ligand-binding regions of the PctA and PctB chemoreceptors from Pseudomonas aeruginosa in complex with amino acids.,Rico-Jimenez M, Munoz-Martinez F, Krell T, Gavira JA, Pineda-Molina E Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Dec;69(Pt 12):1431-5. doi:, 10.1107/S1744309113023592. Epub 2013 Nov 29. PMID:24316847<ref>PMID:24316847</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5t7m" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Chemotaxis protein|Chemotaxis protein]]
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*[[Chemotaxis protein 3D structures|Chemotaxis protein 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Conejero-Muriel, M]]
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[[Category: Large Structures]]
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[[Category: Gavira, J A]]
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[[Category: Pseudomonas aeruginosa]]
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[[Category: Krell, T]]
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[[Category: Conejero-Muriel M]]
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[[Category: Ortega, A]]
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[[Category: Gavira JA]]
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[[Category: Rico-Jimenez, M]]
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[[Category: Krell T]]
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[[Category: Zhulin, I]]
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[[Category: Ortega A]]
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[[Category: Chemotactic transducer]]
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[[Category: Rico-Jimenez M]]
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[[Category: Signaling protein]]
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[[Category: Zhulin I]]

Current revision

LIGAND BINDING DOMAIN OF PSEUDOMONAS AERUGINOSA PAO1 AMINO ACID CHEMORECEPTOR PCTA IN COMPLEX WITH L-TRP

PDB ID 5t7m

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