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| | ==Solution NMR structure of palmitated SCP2L2 from Aedes aegypti== | | ==Solution NMR structure of palmitated SCP2L2 from Aedes aegypti== |
| - | <StructureSection load='2nbn' size='340' side='right' caption='[[2nbn]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2nbn' size='340' side='right'caption='[[2nbn]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2nbn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aedae Aedae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NBN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NBN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2nbn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aedes_aegypti Aedes aegypti]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NBN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NBN FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nbn OCA], [http://pdbe.org/2nbn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2nbn RCSB], [http://www.ebi.ac.uk/pdbsum/2nbn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2nbn ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nbn OCA], [https://pdbe.org/2nbn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nbn RCSB], [https://www.ebi.ac.uk/pdbsum/2nbn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nbn ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q0GY13_AEDAE Q0GY13_AEDAE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aedae]] | |
| - | [[Category: Singarapu, K K]] | |
| - | [[Category: Ummanni, R]] | |
| | [[Category: Aedes aegypti]] | | [[Category: Aedes aegypti]] |
| - | [[Category: Lipid transport]] | + | [[Category: Large Structures]] |
| - | [[Category: Palmitate]] | + | [[Category: Singarapu KK]] |
| - | [[Category: Scp2l2]] | + | [[Category: Ummanni R]] |
| Structural highlights
Function
Q0GY13_AEDAE
Publication Abstract from PubMed
Sterol carrier protein 2 like 2 from Aedes aegypti (AeSCP2L2) plays an important role in lipid transport in mosquitoes for its routine metabolic processes. Repeated unsuccessful attempts to crystallize ligand free SCP2L2 prompted us to undertake nuclear magnetic resonance (NMR) spectroscopy to determine its three-dimensional structure. We report here the three-dimensional structures and dynamics of apo-AeSCP2L2 and its complex with palmitate. The (15)N heteronuclear single-quantum coherence spectrum of apo-AeSCP2L2 displayed multiple peaks for some of the amide resonances, implying the presence of multiple conformations in solution, which are transformed to a single conformation upon formation of the complex with plamitate. The three-dimensional structures of apo-AeSCP2L2 and palmitated AeSCP2L2 reveal an alpha/beta mixed fold, with five beta-strands and four alpha-helices, very similar to the other SCP2 protein structures. Unlike the crystal structure of palmitated AeSCP2L2, both solution structures are monomeric. It is further confirmed by the rotational correlation times determined by NMR relaxation times (T1 and T2) of the amide protons. In addition, the palmitated AeSCP2L2 structure contains two palmitate ligands, bound in the binding pocket, unlike the three palmitates bound in the dimeric form of AeSCP2L2 in the crystals. The relaxation experiments revealed that complex formation significantly reduces the dynamics of the protein in solution.
Solution Nuclear Magnetic Resonance Studies of Sterol Carrier Protein 2 Like 2 (SCP2L2) Reveal the Insecticide Specific Structural Characteristics of SCP2 Proteins in Aedes aegypti Mosquitoes.,Singarapu KK, Ahuja A, Potula PR, Ummanni R Biochemistry. 2016 Sep 6;55(35):4919-27. doi: 10.1021/acs.biochem.6b00322. Epub, 2016 Aug 23. PMID:27508310[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Singarapu KK, Ahuja A, Potula PR, Ummanni R. Solution Nuclear Magnetic Resonance Studies of Sterol Carrier Protein 2 Like 2 (SCP2L2) Reveal the Insecticide Specific Structural Characteristics of SCP2 Proteins in Aedes aegypti Mosquitoes. Biochemistry. 2016 Sep 6;55(35):4919-27. doi: 10.1021/acs.biochem.6b00322. Epub, 2016 Aug 23. PMID:27508310 doi:http://dx.doi.org/10.1021/acs.biochem.6b00322
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