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| | ==Solution NMR Structure of the membrane localization domain from Pasteurella multocida toxin== | | ==Solution NMR Structure of the membrane localization domain from Pasteurella multocida toxin== |
| - | <StructureSection load='2n9v' size='340' side='right' caption='[[2n9v]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2n9v' size='340' side='right'caption='[[2n9v]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2n9v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_multocidum"_lehmann_and_neumann_1899 "bacterium multocidum" lehmann and neumann 1899]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N9V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N9V FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2n9v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N9V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N9V FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2n9w|2n9w]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n9v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n9v OCA], [https://pdbe.org/2n9v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n9v RCSB], [https://www.ebi.ac.uk/pdbsum/2n9v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n9v ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">toxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=747 "Bacterium multocidum" Lehmann and Neumann 1899])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n9v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n9v OCA], [http://pdbe.org/2n9v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n9v RCSB], [http://www.ebi.ac.uk/pdbsum/2n9v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n9v ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TOXA_PASMD TOXA_PASMD]] This is a dermonecrotic toxin. This osteolytic toxin, induces bone resorption. Potent mitogen. This toxin is associated with the severe progressive form of the atrophic rhinitis, a major respiratory disease in pigs. | + | [https://www.uniprot.org/uniprot/TOXA_PASMD TOXA_PASMD] This is a dermonecrotic toxin. This osteolytic toxin, induces bone resorption. Potent mitogen. This toxin is associated with the severe progressive form of the atrophic rhinitis, a major respiratory disease in pigs. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacterium multocidum lehmann and neumann 1899]] | + | [[Category: Large Structures]] |
| - | [[Category: Brothers, M C]] | + | [[Category: Pasteurella multocida]] |
| - | [[Category: Hisao, G S]] | + | [[Category: Brothers MC]] |
| - | [[Category: Ho, M]] | + | [[Category: Hisao GS]] |
| - | [[Category: Rienstra, C M]] | + | [[Category: Ho M]] |
| - | [[Category: Wilson, B A]] | + | [[Category: Rienstra CM]] |
| - | [[Category: Bacterial toxin]] | + | [[Category: Wilson BA]] |
| - | [[Category: Membrane localization domain]]
| + | |
| - | [[Category: Toxin]]
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| Structural highlights
Function
TOXA_PASMD This is a dermonecrotic toxin. This osteolytic toxin, induces bone resorption. Potent mitogen. This toxin is associated with the severe progressive form of the atrophic rhinitis, a major respiratory disease in pigs.
Publication Abstract from PubMed
Membrane localization domain (MLD) was first proposed for a 4-helix-bundle motif in the crystal structure of the C1 domain of Pasteurella multocida toxin (PMT). This structure motif is also found in the crystal structures of several clostridial glycosylating toxins (TcdA, TcdB, TcsL, and TcnA). The Ras/Rap1-specific endopeptidase (RRSP) module of the multifunctional autoprocessing repeats-in-toxins (MARTX) toxin produced by Vibrio vulnificus has sequence homology to the C1-C2 domains of PMT, including a putative MLD. We have determined the solution structure for the MLDs in PMT and in RRSP using solution state NMR. We conclude that the MLDs in these two toxins assume a 4-helix-bundle structure in solution. This article is protected by copyright. All rights reserved.
The membrane localization domains of two distinct bacterial toxins form a 4-helix-bundle in solution.,Hisao GS, Brothers MC, Ho M, Wilson BA, Rienstra CM Protein Sci. 2016 Dec 15. doi: 10.1002/pro.3097. PMID:27977897[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hisao GS, Brothers MC, Ho M, Wilson BA, Rienstra CM. The membrane localization domains of two distinct bacterial toxins form a 4-helix-bundle in solution. Protein Sci. 2016 Dec 15. doi: 10.1002/pro.3097. PMID:27977897 doi:http://dx.doi.org/10.1002/pro.3097
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