|
|
| (One intermediate revision not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Solution structure of ZitP zinc finger== | | ==Solution structure of ZitP zinc finger== |
| - | <StructureSection load='2nb9' size='340' side='right' caption='[[2nb9]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2nb9' size='340' side='right'caption='[[2nb9]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2nb9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cauvc Cauvc]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NB9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NB9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2nb9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caulobacter_vibrioides_CB15 Caulobacter vibrioides CB15]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NB9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NB9 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CC_2215 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=190650 CAUVC])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nb9 OCA], [https://pdbe.org/2nb9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nb9 RCSB], [https://www.ebi.ac.uk/pdbsum/2nb9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nb9 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nb9 OCA], [http://pdbe.org/2nb9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2nb9 RCSB], [http://www.ebi.ac.uk/pdbsum/2nb9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2nb9 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9A679_CAUVC Q9A679_CAUVC] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 21: |
Line 22: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cauvc]] | + | [[Category: Caulobacter vibrioides CB15]] |
| - | [[Category: Allain, F H.T]] | + | [[Category: Large Structures]] |
| - | [[Category: Berge, M]] | + | [[Category: Allain FH-T]] |
| - | [[Category: Campagne, S]] | + | [[Category: Berge M]] |
| - | [[Category: Viollier, P H]] | + | [[Category: Campagne S]] |
| - | [[Category: Unknown function]]
| + | [[Category: Viollier PH]] |
| - | [[Category: Zinc finger]]
| + | |
| Structural highlights
Function
Q9A679_CAUVC
Publication Abstract from PubMed
Although free-living and obligate intracellular bacteria are both polarized it is unclear whether the underlying polarization mechanisms and effector proteins are conserved. Here we dissect at the cytological, functional and structural level a conserved polarization module from the free living alpha-proteobacterium Caulobacter crescentus and an orthologous system from an obligate intracellular (rickettsial) pathogen. The NMR solution structure of the zinc-finger (ZnR) domain from the bifunctional and bipolar ZitP pilus assembly/motility regulator revealed conserved interaction determinants for PopZ, a bipolar matrix protein that anchors the ParB centromere-binding protein and other regulatory factors at the poles. We show that ZitP regulates cytokinesis and the localization of ParB and PopZ, targeting PopZ independently of the previously known binding sites for its client proteins. Through heterologous localization assays with rickettsial ZitP and PopZ orthologs, we document the shared ancestries, activities and structural determinants of a (bi-)polarization system encoded in free-living and obligate intracellular alpha-proteobacteria.
Modularity and determinants of a (bi-)polarization control system from free-living and obligate intracellular bacteria.,Berge M, Campagne S, Mignolet J, Holden S, Theraulaz L, Manley S, Allain FH, Viollier PH Elife. 2016 Dec 23;5. pii: e20640. doi: 10.7554/eLife.20640. PMID:28008852[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Berge M, Campagne S, Mignolet J, Holden S, Theraulaz L, Manley S, Allain FH, Viollier PH. Modularity and determinants of a (bi-)polarization control system from free-living and obligate intracellular bacteria. Elife. 2016 Dec 23;5. pii: e20640. doi: 10.7554/eLife.20640. PMID:28008852 doi:http://dx.doi.org/10.7554/eLife.20640
|
