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| | ==Structure of CrgA, a LysR-type transcriptional regulator from Neisseria meningitidis.== | | ==Structure of CrgA, a LysR-type transcriptional regulator from Neisseria meningitidis.== |
| - | <StructureSection load='3hhg' size='340' side='right' caption='[[3hhg]], [[Resolution|resolution]] 3.20Å' scene=''> | + | <StructureSection load='3hhg' size='340' side='right'caption='[[3hhg]], [[Resolution|resolution]] 3.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3hhg]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Neimi Neimi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HHG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HHG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3hhg]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_meningitidis_serogroup_B Neisseria meningitidis serogroup B]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HHG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HHG FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hhf|3hhf]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CrgA, NMB1856 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=491 NEIMI])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hhg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hhg OCA], [https://pdbe.org/3hhg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hhg RCSB], [https://www.ebi.ac.uk/pdbsum/3hhg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hhg ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hhg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hhg OCA], [http://pdbe.org/3hhg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hhg RCSB], [http://www.ebi.ac.uk/pdbsum/3hhg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3hhg ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9JXW7_NEIMB Q9JXW7_NEIMB] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </div> | | </div> |
| | <div class="pdbe-citations 3hhg" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 3hhg" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Neimi]] | + | [[Category: Large Structures]] |
| - | [[Category: OPPF, Oxford Protein Production Facility]] | + | [[Category: Neisseria meningitidis serogroup B]] |
| - | [[Category: Owens, R J]] | + | [[Category: Owens RJ]] |
| - | [[Category: Ren, J]] | + | [[Category: Ren J]] |
| - | [[Category: Sainsbury, S]] | + | [[Category: Sainsbury S]] |
| - | [[Category: Stuart, D I]] | + | [[Category: Stuart DI]] |
| - | [[Category: Dna-binding]]
| + | |
| - | [[Category: Lysr]]
| + | |
| - | [[Category: Neisseria meningitidi]]
| + | |
| - | [[Category: Oppf]]
| + | |
| - | [[Category: Oxford protein production facility]]
| + | |
| - | [[Category: Structural genomic]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcription factor]]
| + | |
| - | [[Category: Transcription regulation]]
| + | |
| - | [[Category: Transcription regulator]]
| + | |
| Structural highlights
Function
Q9JXW7_NEIMB
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
LysR-type transcriptional regulators (LTTRs) form the largest family of bacterial regulators acting as both auto-repressors and activators of target promoters, controlling operons involved in a wide variety of cellular processes. The LTTR, CrgA, from the human pathogen Neisseria meningitidis, is upregulated during bacterial-host cell contact. Here, we report the crystal structures of both regulatory domain and full-length CrgA, the first of a novel subclass of LTTRs that form octameric rings. Non-denaturing mass spectrometry analysis and analytical ultracentrifugation established that the octameric form of CrgA is the predominant species in solution in both the presence and absence of an oligonucleotide encompassing the CrgA-binding sequence. Furthermore, analysis of the isolated CrgA-DNA complex by mass spectrometry showed stabilization of a double octamer species upon DNA binding. Based on the observed structure and the mass spectrometry findings, a model is proposed in which a hexadecameric array of two CrgA oligomers binds to its DNA target site.
The structure of CrgA from Neisseria meningitidis reveals a new octameric assembly state for LysR transcriptional regulators.,Sainsbury S, Lane LA, Ren J, Gilbert RJ, Saunders NJ, Robinson CV, Stuart DI, Owens RJ Nucleic Acids Res. 2009 Aug;37(14):4545-58. Epub 2009 May 27. PMID:19474343[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Sainsbury S, Lane LA, Ren J, Gilbert RJ, Saunders NJ, Robinson CV, Stuart DI, Owens RJ. The structure of CrgA from Neisseria meningitidis reveals a new octameric assembly state for LysR transcriptional regulators. Nucleic Acids Res. 2009 Aug;37(14):4545-58. Epub 2009 May 27. PMID:19474343 doi:10.1093/nar/gkp445
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