2c4b

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:48, 23 October 2024) (edit) (undo)
 
(20 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2c4b.gif|left|200px]]<br />
 
-
<applet load="2c4b" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2c4b, resolution 1.30&Aring;" />
 
-
'''INHIBITOR CYSTINE KNOT PROTEIN MCOEETI FUSED TO THE CATALYTICALLY INACTIVE BARNASE MUTANT H102A'''<br />
 
-
==Overview==
+
==Inhibitor cystine knot protein McoEeTI fused to the catalytically inactive barnase mutant H102A==
-
We present a fusion system suited to determine the crystal structure of, small disulfide-rich proteins. McoEeTI, a hybrid inhibitor cystine knot, microprotein, was produced as a soluble fusion to a catalytically inactive, variant of the RNAse barnase in Escherichia coli. Functioning as a, versatile tag, barnase facilitated purification, crystallization and, high-resolution structure determination. Flexibility of the linker region, allows for different relative orientations of barnase and the fusion, partner in two crystallographically independent molecules and may thereby, facilitate crystal packing. Nevertheless, the linker region is well, ordered in both molecules. This system may prove more generally useful to, determine the crystal structure of peptides and small proteins.
+
<StructureSection load='2c4b' size='340' side='right'caption='[[2c4b]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2c4b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens], [https://en.wikipedia.org/wiki/Ecballium_elaterium Ecballium elaterium] and [https://en.wikipedia.org/wiki/Momordica_cochinchinensis Momordica cochinchinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C4B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C4B FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c4b OCA], [https://pdbe.org/2c4b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c4b RCSB], [https://www.ebi.ac.uk/pdbsum/2c4b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c4b ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RNBR_BACAM RNBR_BACAM] Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates.[https://www.uniprot.org/uniprot/ITR2_ECBEL ITR2_ECBEL] Inhibits trypsin.[https://www.uniprot.org/uniprot/ITR2_MOMCO ITR2_MOMCO]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c4/2c4b_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2c4b ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
We present a fusion system suited to determine the crystal structure of small disulfide-rich proteins. McoEeTI, a hybrid inhibitor cystine knot microprotein, was produced as a soluble fusion to a catalytically inactive variant of the RNAse barnase in Escherichia coli. Functioning as a versatile tag, barnase facilitated purification, crystallization and high-resolution structure determination. Flexibility of the linker region allows for different relative orientations of barnase and the fusion partner in two crystallographically independent molecules and may thereby facilitate crystal packing. Nevertheless, the linker region is well ordered in both molecules. This system may prove more generally useful to determine the crystal structure of peptides and small proteins.
-
==About this Structure==
+
Barnase fusion as a tool to determine the crystal structure of the small disulfide-rich protein McoEeTI.,Niemann HH, Schmoldt HU, Wentzel A, Kolmar H, Heinz DW J Mol Biol. 2006 Feb 10;356(1):1-8. Epub 2005 Nov 21. PMID:16337652<ref>PMID:16337652</ref>
-
2C4B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens,_momordica_cochinchinensis,_ecballium_elaterium Bacillus amyloliquefaciens, momordica cochinchinensis, ecballium elaterium] with SO4, 2PE, EDO, MES, UNX, GOL and FMT as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C4B OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Barnase fusion as a tool to determine the crystal structure of the small disulfide-rich protein McoEeTI., Niemann HH, Schmoldt HU, Wentzel A, Kolmar H, Heinz DW, J Mol Biol. 2006 Feb 10;356(1):1-8. Epub 2005 Nov 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16337652 16337652]
+
</div>
-
[[Category: Bacillus amyloliquefaciens, momordica cochinchinensis, ecballium elaterium]]
+
<div class="pdbe-citations 2c4b" style="background-color:#fffaf0;"></div>
-
[[Category: Ribonuclease T(1)]]
+
-
[[Category: Single protein]]
+
-
[[Category: Heinz, D.W.]]
+
-
[[Category: Kolmar, H.]]
+
-
[[Category: Niemann, H.H.]]
+
-
[[Category: Schmoldt, H.U.]]
+
-
[[Category: Wentzel, A.]]
+
-
[[Category: 2PE]]
+
-
[[Category: EDO]]
+
-
[[Category: FMT]]
+
-
[[Category: GOL]]
+
-
[[Category: MES]]
+
-
[[Category: SO4]]
+
-
[[Category: UNX]]
+
-
[[Category: endonuclease]]
+
-
[[Category: fusion protein]]
+
-
[[Category: hybrid microprotein]]
+
-
[[Category: hydrolase]]
+
-
[[Category: nuclease]]
+
-
[[Category: protease inhibitor]]
+
-
[[Category: ribonuclease]]
+
-
[[Category: serine protease inhibitor]]
+
-
[[Category: squash inhibitor]]
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 17:58:14 2007''
+
==See Also==
 +
*[[Barnase 3D structures|Barnase 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Bacillus amyloliquefaciens]]
 +
[[Category: Ecballium elaterium]]
 +
[[Category: Large Structures]]
 +
[[Category: Momordica cochinchinensis]]
 +
[[Category: Heinz DW]]
 +
[[Category: Kolmar H]]
 +
[[Category: Niemann HH]]
 +
[[Category: Schmoldt HU]]
 +
[[Category: Wentzel A]]

Current revision

Inhibitor cystine knot protein McoEeTI fused to the catalytically inactive barnase mutant H102A

PDB ID 2c4b

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2c4b"
Personal tools