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6i2z
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Isolated globin domain of the Bordetella pertussis globin-coupled sensor== | |
| + | <StructureSection load='6i2z' size='340' side='right'caption='[[6i2z]], [[Resolution|resolution]] 3.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6i2z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bordetella_pertussis_Tohama_I Bordetella pertussis Tohama I]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I2Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6I2Z FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6i2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i2z OCA], [https://pdbe.org/6i2z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6i2z RCSB], [https://www.ebi.ac.uk/pdbsum/6i2z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6i2z ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q7VTL8_BORPE Q7VTL8_BORPE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | AIMS: Structural and functional characterization of the globin coupled sensors (GCS) of <i>Azotobacter vinelandii </i>(AvGReg) and <i>Bordetella pertussis</i> (BpeGReg). RESULTS: UV/Vis and RR spectroscopies confirm the presence in <i>Av</i>GReg and <i>Bpe</i>GReg of a globin domain capable of reversible gaseous ligand binding. In <i>Av</i>GReg an influence of the transmitter domain on the heme proximal region of the globin domain can be seen, and <i>k'</i>CO is higher than for other GCSs. The O<sub>2</sub> binding kinetics suggest the presence of an open and a closed conformation. As for <i>Bpe</i>GReg, the fully oxygenated <i>Av</i>GReg show a very high DGC activity. The CO rebinding to <i>Bpe</i>GReg indicates that intra- and intermolecular interactions influence the ligand binding. The globin domains of both proteins (<i>Av</i>GReg-Gb and <i>Bpe</i>Greg-Gb*) share the same GCS fold, a similar proximal but a different distal side structure. They homodimerize through a G-H helical bundle as in other GCS. However, <i>Bpe</i>GReg-Gb* shows also a second dimerization mode. INNOVATION: This paper extends our knowledge on the GCS proteins and contributes to a better understanding of the GCSs' role in the formation of bacterial biofilms. CONCLUSIONS: <i>Av</i>GReg and <i>Bpe</i>Greg conform to the GCS family, share a similar overall structure but they have different properties in terms of the ligand binding. In particular, <i>Av</i>Greg shows an open and closed conformation that in the latter form will very tightly bind oxygen. <i>Bpe</i>GReg has only one closed conformation. In both proteins, it is the fully oxygenated GCS form that catalyzes the production of the second messenger. | ||
| - | + | Structural and functional characterization of the globin-coupled sensors of <i>Azotobacter vinelandii </i>and <i>Bordetella pertussis</i>.,Germani F, Nardini M, De Schutter A, Cuypers B, Berghmans H, Van Hauwaert ML, Stefano B, Mozzarelli A, Moens L, Van Doorslaer S, Bolognesi M, Pesce A, Dewilde S Antioxid Redox Signal. 2019 Sep 27. doi: 10.1089/ars.2018.7690. PMID:31559835<ref>PMID:31559835</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6i2z" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: | + | [[Category: Bordetella pertussis Tohama I]] |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Berghmans H]] |
| - | [[Category: | + | [[Category: Bolognesi M]] |
| - | [[Category: | + | [[Category: Bruno S]] |
| - | [[Category: Van | + | [[Category: Cuypers B]] |
| - | [[Category: | + | [[Category: De Schutter A]] |
| + | [[Category: Dewilde S]] | ||
| + | [[Category: Germani F]] | ||
| + | [[Category: Moens L]] | ||
| + | [[Category: Mozzarelli A]] | ||
| + | [[Category: Pesce A]] | ||
| + | [[Category: Van Doorslaer S]] | ||
| + | [[Category: Van Hauwaert M-L]] | ||
Current revision
Isolated globin domain of the Bordetella pertussis globin-coupled sensor
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