6nc8
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Lipid II flippase MurJ, inward occluded conformation== | |
| + | <StructureSection load='6nc8' size='340' side='right'caption='[[6nc8]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6nc8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosipho_africanus_TCF52B Thermosipho africanus TCF52B]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NC8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NC8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.601Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nc8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nc8 OCA], [https://pdbe.org/6nc8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nc8 RCSB], [https://www.ebi.ac.uk/pdbsum/6nc8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nc8 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/MURJ_THEAB MURJ_THEAB] Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane.[HAMAP-Rule:MF_02078]<ref>PMID:28024149</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The biosynthesis of many polysaccharides, including bacterial peptidoglycan and eukaryotic N-linked glycans, requires transport of lipid-linked oligosaccharide (LLO) precursors across the membrane by specialized flippases. MurJ is the flippase for the lipid-linked peptidoglycan precursor Lipid II, a key player in bacterial cell wall synthesis, and a target of recently discovered antibacterials. However, the flipping mechanism of LLOs including Lipid II remains poorly understood due to a dearth of structural information. Here we report crystal structures of MurJ captured in inward-closed, inward-open, inward-occluded and outward-facing conformations. Together with mutagenesis studies, we elucidate the conformational transitions in MurJ that mediate lipid flipping, identify the key ion for function, and provide a framework for the development of inhibitors. | ||
| - | + | Visualizing conformation transitions of the Lipid II flippase MurJ.,Kuk ACY, Hao A, Guan Z, Lee SY Nat Commun. 2019 Apr 15;10(1):1736. doi: 10.1038/s41467-019-09658-0. PMID:30988294<ref>PMID:30988294</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6nc8" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Thermosipho africanus TCF52B]] | ||
| + | [[Category: Kuk ACY]] | ||
| + | [[Category: Lee S-Y]] | ||
Current revision
Lipid II flippase MurJ, inward occluded conformation
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