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| | ==Crystal Structure of IclR Family Transcriptional Regulator from Brucella abortus== | | ==Crystal Structure of IclR Family Transcriptional Regulator from Brucella abortus== |
| - | <StructureSection load='5whm' size='340' side='right' caption='[[5whm]], [[Resolution|resolution]] 1.95Å' scene=''> | + | <StructureSection load='5whm' size='340' side='right'caption='[[5whm]], [[Resolution|resolution]] 1.95Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5whm]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_of_abortion"_bang_1897 "bacillus of abortion" bang 1897]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WHM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WHM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5whm]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Brucella_abortus Brucella abortus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WHM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WHM FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BFJ58_02720 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=235 "Bacillus of abortion" Bang 1897])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5whm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5whm OCA], [https://pdbe.org/5whm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5whm RCSB], [https://www.ebi.ac.uk/pdbsum/5whm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5whm ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5whm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5whm OCA], [http://pdbe.org/5whm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5whm RCSB], [http://www.ebi.ac.uk/pdbsum/5whm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5whm ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus of abortion bang 1897]] | + | [[Category: Brucella abortus]] |
| - | [[Category: Babnigg, G]] | + | [[Category: Large Structures]] |
| - | [[Category: Crosson, S]] | + | [[Category: Babnigg G]] |
| - | [[Category: Endres, M]] | + | [[Category: Crosson S]] |
| - | [[Category: Joachimiak, A]] | + | [[Category: Endres M]] |
| - | [[Category: Kim, Y]] | + | [[Category: Joachimiak A]] |
| - | [[Category: Structural genomic]]
| + | [[Category: Kim Y]] |
| - | [[Category: Tesar, C]] | + | [[Category: Tesar C]] |
| - | [[Category: Wu, R]] | + | [[Category: Wu R]] |
| - | [[Category: Chicago center for functional annotation]]
| + | |
| - | [[Category: Helix-turn-helix]]
| + | |
| - | [[Category: Mcsg]]
| + | |
| - | [[Category: Psi-biology]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcription factor]]
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| Structural highlights
Publication Abstract from PubMed
The general stress response sigma factor sigma(E1) directly and indirectly regulates the transcription of dozens of genes that influence stress survival and host infection in the zoonotic pathogen Brucella abortus Characterizing the functions of sigma(E1)-regulated genes therefore would contribute to our understanding of B. abortus physiology and infection biology. sigma(E1) indirectly activates transcription of the IclR family regulator Bab2_0215, but the function of this regulator remains undefined. Here, we present a structural and functional characterization of Bab2_0215, which we have named B rucella adipic acid-activated regulator (BaaR). We found that BaaR adopts a classic IclR-family fold and directly represses the transcription of two operons with predicted roles in carboxylic acid oxidation. BaaR binds two sites on chromosome II between baaR and a divergently transcribed hydratase/dehydrogenase (acaD2), and it represses transcription of both genes. We identified three carboxylic acids (adipic acid, tetradecanedioic acid, and -aminocaproic acid) and a lactone (-caprolactone) that enhance transcription from the baaR and acaD2 promoters. However, neither the activating acids nor caprolactone enhanced transcription by binding directly to BaaR. Induction of baaR transcription by adipic acid required the gene bab2_0213, which encodes a major facilitator superfamily transporter, suggesting that Bab2_0213 transports adipic acid across the inner membrane. We conclude that a suite of structurally related organic molecules activate transcription of genes repressed by BaaR. Our study provides molecular-level understanding of a gene expression program in B. abortus that is downstream of sigma(E1).
Molecular control of gene expression by Brucella BaaR, an IclR-type transcriptional repressor.,Herrou J, Czyz DM, Fiebig A, Willett JW, Kim Y, Wu R, Babnigg G, Crosson S J Biol Chem. 2018 May 11;293(19):7437-7456. doi: 10.1074/jbc.RA118.002045. Epub, 2018 Mar 22. PMID:29567835[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Herrou J, Czyz DM, Fiebig A, Willett JW, Kim Y, Wu R, Babnigg G, Crosson S. Molecular control of gene expression by Brucella BaaR, an IclR-type transcriptional repressor. J Biol Chem. 2018 May 11;293(19):7437-7456. doi: 10.1074/jbc.RA118.002045. Epub, 2018 Mar 22. PMID:29567835 doi:http://dx.doi.org/10.1074/jbc.RA118.002045
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