|
|
| (One intermediate revision not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==apPEP_D622N opened state== | | ==apPEP_D622N opened state== |
| - | <StructureSection load='3iun' size='340' side='right' caption='[[3iun]], [[Resolution|resolution]] 2.40Å' scene=''> | + | <StructureSection load='3iun' size='340' side='right'caption='[[3iun]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3iun]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"aeromonas_caviae"_eddy_1962 "aeromonas caviae" eddy 1962]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IUN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IUN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3iun]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeromonas_caviae Aeromonas caviae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IUN FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3iuj|3iuj]], [[3iul|3iul]], [[3ium|3ium]], [[3iuq|3iuq]], [[3iur|3iur]], [[3ivm|3ivm]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">prolyl endopeptidase ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=648 "Aeromonas caviae" Eddy 1962])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3iun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iun OCA], [https://pdbe.org/3iun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3iun RCSB], [https://www.ebi.ac.uk/pdbsum/3iun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3iun ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iun OCA], [http://pdbe.org/3iun PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3iun RCSB], [http://www.ebi.ac.uk/pdbsum/3iun PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3iun ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9X6R4_AERCA Q9X6R4_AERCA] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| Line 35: |
Line 36: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aeromonas caviae eddy 1962]] | + | [[Category: Aeromonas caviae]] |
| - | [[Category: Chiu, T K]] | + | [[Category: Large Structures]] |
| - | [[Category: Hydrolase]] | + | [[Category: Chiu TK]] |
| - | [[Category: Prolyl endopeptidase]]
| + | |
| Structural highlights
Function
Q9X6R4_AERCA
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Prolyl peptidases cleave proteins at proline residues and are of importance for cancer, neurological function, and type II diabetes. Prolyl endopeptidase (PEP) cleaves neuropeptides and is a drug target for neuropsychiatric diseases such as post-traumatic stress disorder, depression, and schizophrenia. Previous structural analyses showing little differences between native and substrate-bound structures have suggested a lock-and-key catalytic mechanism. We now directly demonstrate from seven structures of Aeromonus punctata PEP that the mechanism is instead induced fit: the native enzyme exists in a conformationally flexible opened state with a large interdomain opening between the beta-propeller and alpha/beta-hydrolase domains; addition of substrate to preformed native crystals induces a large scale conformational change into a closed state with induced-fit adjustments of the active site, and inhibition of this conformational change prevents substrate binding. Absolute sequence conservation among 28 orthologs of residues at the active site and critical residues at the interdomain interface indicates that this mechanism is conserved in all PEPs. This finding has immediate implications for the use of conformationally targeted drug design to improve specificity of inhibition against this family of proline-specific serine proteases.
Induced-fit mechanism for prolyl endopeptidase.,Li M, Chen C, Davies DR, Chiu TK J Biol Chem. 2010 Jul 9;285(28):21487-95. Epub 2010 May 5. PMID:20444688[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Li M, Chen C, Davies DR, Chiu TK. Induced-fit mechanism for prolyl endopeptidase. J Biol Chem. 2010 Jul 9;285(28):21487-95. Epub 2010 May 5. PMID:20444688 doi:10.1074/jbc.M109.092692
|
