3cfe

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[[Image:3cfe.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of purple-fluorescent antibody EP2-25C10==
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|PDB= 3cfe |SIZE=350|CAPTION= <scene name='initialview01'>3cfe</scene>, resolution 2.99&Aring;
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<StructureSection load='3cfe' size='340' side='right'caption='[[3cfe]], [[Resolution|resolution]] 2.99&Aring;' scene=''>
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|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+A+301'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Residue+L+302'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Residue+L+303'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Residue+A+304'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Residue+B+305'>AC5</scene>, <scene name='pdbsite=AC6:Gol+Binding+Site+For+Residue+H+401'>AC6</scene>, <scene name='pdbsite=AC7:Gol+Binding+Site+For+Residue+L+402'>AC7</scene> and <scene name='pdbsite=AC8:Gol+Binding+Site+For+Residue+A+403'>AC8</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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<table><tr><td colspan='2'>[[3cfe]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CFE FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.99&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cfe OCA], [https://pdbe.org/3cfe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cfe RCSB], [https://www.ebi.ac.uk/pdbsum/3cfe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cfe ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1fl3|1FL3]], [[1ub5|1UB5]], [[1ub6|1UB6]], [[3cfb|3CFB]], [[3cfc|3CFC]], [[3cfd|3CFD]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cfe OCA], [http://www.ebi.ac.uk/pdbsum/3cfe PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3cfe RCSB]</span>
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== Evolutionary Conservation ==
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}}
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/3cfe_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cfe ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The blue-emissive antibody EP2-19G2 that has been elicited against trans-stilbene has unprecedented ability to produce bright luminescence and has been used as a biosensor in various applications. We show that the prolonged luminescence is not stilbene fluorescence. Instead, the emissive species is a charge-transfer excited complex of an anionic stilbene and a cationic, parallel pi-stacked tryptophan. Upon charge recombination, this complex generates exceptionally bright blue light. Complex formation is enabled by a deeply penetrating ligand-binding pocket, which in turn results from a noncanonical interface between the two variable domains of the antibody.
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'''Crystal structure of purple-fluorescent antibody EP2-25C10'''
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Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex.,Debler EW, Kaufmann GF, Meijler MM, Heine A, Mee JM, Pljevaljcic G, Di Bilio AJ, Schultz PG, Millar DP, Janda KD, Wilson IA, Gray HB, Lerner RA Science. 2008 Feb 29;319(5867):1232-5. PMID:18309081<ref>PMID:18309081</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3cfe" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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The blue-emissive antibody EP2-19G2 that has been elicited against trans-stilbene has unprecedented ability to produce bright luminescence and has been used as a biosensor in various applications. We show that the prolonged luminescence is not stilbene fluorescence. Instead, the emissive species is a charge-transfer excited complex of an anionic stilbene and a cationic, parallel pi-stacked tryptophan. Upon charge recombination, this complex generates exceptionally bright blue light. Complex formation is enabled by a deeply penetrating ligand-binding pocket, which in turn results from a noncanonical interface between the two variable domains of the antibody.
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[Sandbox 20009|Sandbox 20009]]
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==About this Structure==
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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3CFE is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFE OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex., Debler EW, Kaufmann GF, Meijler MM, Heine A, Mee JM, Pljevaljcic G, Di Bilio AJ, Schultz PG, Millar DP, Janda KD, Wilson IA, Gray HB, Lerner RA, Science. 2008 Feb 29;319(5867):1232-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18309081 18309081]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Debler EW]]
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[[Category: Debler, E W.]]
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[[Category: Heine A]]
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[[Category: Heine, A.]]
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[[Category: Wilson IA]]
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[[Category: Wilson, I A.]]
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[[Category: hapten complex]]
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[[Category: immune system]]
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[[Category: immunoglobulin]]
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[[Category: purple-fluorescent antibody]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:32:02 2008''
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Current revision

Crystal structure of purple-fluorescent antibody EP2-25C10

PDB ID 3cfe

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