5b61

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==Extra-superfolder GFP==
==Extra-superfolder GFP==
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<StructureSection load='5b61' size='340' side='right' caption='[[5b61]], [[Resolution|resolution]] 3.12&Aring;' scene=''>
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<StructureSection load='5b61' size='340' side='right'caption='[[5b61]], [[Resolution|resolution]] 3.12&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5b61]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B61 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B61 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5b61]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B61 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5B61 FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gfp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.115&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b61 OCA], [http://pdbe.org/5b61 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b61 RCSB], [http://www.ebi.ac.uk/pdbsum/5b61 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5b61 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5b61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b61 OCA], [https://pdbe.org/5b61 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5b61 RCSB], [https://www.ebi.ac.uk/pdbsum/5b61 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5b61 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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Stability of green fluorescent protein (GFP) is sometimes important for a proper practical application of this protein. Random mutagenesis and targeted mutagenesis have been used to create better-folded variants of GFP, including recently reported extra-superfolder GFP. Our aim was to determine the crystal structure of extra-superfolder GFP, which is more robustly folded and stable than GFP and superfolder GFP. The structural and structure-based mutagenesis analyses revealed that some of the mutations that created extra-superfolder GFP (F46L, E126K, N149K, and S208L) contribute to folding robustness by stabilizing extra-superfolder GFP with various noncovalent bonds.
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The mechanism of folding robustness revealed by the crystal structure of extra-superfolder GFP.,Choi JY, Jang TH, Park HH FEBS Lett. 2017 Jan;591(2):442-447. doi: 10.1002/1873-3468.12534. Epub 2016 Dec, 28. PMID:27990640<ref>PMID:27990640</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5b61" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Green Fluorescent Protein|Green Fluorescent Protein]]
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Aeqvi]]
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[[Category: Aequorea victoria]]
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[[Category: Choi, J Y]]
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[[Category: Large Structures]]
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[[Category: Jang, T H]]
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[[Category: Choi JY]]
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[[Category: Park, H H]]
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[[Category: Jang T-H]]
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[[Category: Fluorescent protein]]
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[[Category: Park HH]]
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[[Category: Folding robustness]]
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[[Category: Gfp]]
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[[Category: Random mutagenesis]]
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Current revision

Extra-superfolder GFP

PDB ID 5b61

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